Effects of sepsis on the metabolism of sphingomyelin and cholesterol in mice with liver dysfunction

Experimental and Therapeutic Medicine
Jiaqi LiNianlong Yan

Abstract

Sepsis is characterized by a severe inflammatory response to infection. With the spread of sepsis, various tissues, including the lungs, liver and kidney, may be damaged. This may finally develop into multiple organ dysfunction syndrome. Sphingomyelin and cholesterol are two main lipids involved in sepsis. The metabolism of sphingomyelin and cholesterol in the livers of mice with sepsis needs to be clarified. To achieve this, the present study intraperitoneally injected mice with PBS, lipopolysaccharide (LPS; 10 mg/kg) and LPS + pyrrolidine dithiocarbamate (PDTC; 30 mg/kg). Subsequently, sphingomyelin and cholesterol content were measured using kits, the sphingomyelin synthase (SMS) activity was measured using thin layer chromatography, and the expression levels of SMS1 and 2, hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), ATP binding cassette subfamily A member 1 (ABCA1), scavenger receptor class B member 1 (SR-B1) and apolipoprotein A1 (Apo A1) were determined by western blotting in the livers of mice. Results demonstrated that, in the LPS group, sphingomyelin and cholesterol content was significantly increased (P<0.001; n=6), the SMS activity significantly enhanced (P<0.001; n=6), the expression levels of SMS2, HMGCR...Continue Reading

References

Jun 5, 1997·Nature·K Simons, E Ikonen
Jan 30, 2002·British Journal of Pharmacology·Salvatore CuzzocreaChristoph Thiemermann
Mar 17, 2004·American Journal of Respiratory and Critical Care Medicine·Xinqi PengJoe G N Garcia
Aug 4, 2004·Circulation·Yaniv AlmogAbraham Danon
Jun 25, 2005·Circulation Research·Gary F Lewis, Daniel J Rader
Jun 21, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Tiruneh K HailemariamXian-Cheng Jiang
Mar 17, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Jing LiuXian-Cheng Jiang
Jan 26, 2010·The Journal of Biological Chemistry·Meryem BektasRichard L Proia
Dec 31, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Satish GowdaRaj Wadgaonkar
Feb 3, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ping-Chih HoLi-Na Wei
Jan 1, 1992·Restorative Neurology and Neuroscience·J C CasselB E Will
Aug 13, 2011·Nature·Janet C Garber
Dec 16, 2011·Nutrition & Metabolism·Calvin YeangXian-Cheng Jiang
Mar 1, 2012·American Journal of Respiratory Cell and Molecular Biology·Fatima AnjumRaj Wadgaonkar
Jun 28, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Soonkyu ChungJohn S Parks
Sep 2, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Sahin KabayCansu Ozbayer
Dec 3, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Xin-Chuan ChenXin-Zu Chen
Aug 19, 2015·Clinical and Investigative Medicine. Médecine Clinique Et Experimentale·Ibrahim M OzgulerHandan Akbulut

❮ Previous
Next ❯

Citations

Sep 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiatian LiNianlong Yan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.