Abstract
The role of metabolic disorders of long-chain fatty acid oxidation in the development of pre-eclampsia (PE), hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome and antiphospholipid syndrome (APS) is unclear. The aim of this study was to research the effects of three serum on fatty acid oxidation in trophoblast cells. Primary human trophoblast cells and HTR8/SVneo cells were treated with serum from patients with early-onset severe PE (E-PE), E-PE with HELLP (PE-HELLP), APS, and normal pregnant women as controls (NC). Cells treated with free fatty acids (FFAs) of various lengths were used as controls. Triglyceride (TG) and FFA levels of the E-PE and APS groups were significantly higher than the PE-HELLP and NC groups (P < 0.05). Trophoblast cells treated with serum from the E-PE and APS groups showed obvious morphological changes and a large amount of lipid droplet deposition. Cells in the E-PE group had more severe damage of mitochondria ultrastructure, presenting with cell morphological changes, lipid droplet deposition, and mitochondria damage similar to the long-chain FFA group. FFA levels in the PE-HELLP group increased significantly compared with the NC group (P < 0.05), while TG levels did not change sig...Continue Reading
References
Jun 1, 1993·Experimental Cell Research·C H GrahamP K Lala
Feb 13, 2003·American Journal of Physiology. Endocrinology and Metabolism·Prem ShekhawatArnold W Strauss
Jul 5, 2003·Placenta·C W G Redman, I L Sargent
Nov 18, 2003·Reproductive Biology and Endocrinology : RB&E·Michael J Kupferminc
May 4, 2004·Obstetrics and Gynecology·Ingrit G I ThieleJan G Aarnoudse
Dec 14, 2004·Clinical Rheumatology·Martial KoenigPascal Cathébras
Feb 13, 2007·Clinical Biochemistry·Mirjana BećarevićNada Majkić-Singh
May 29, 2007·Current Rheumatology Reports·Erin A S ClarkD Ware Branch
Jun 20, 2007·Rheumatology International·Panagiotis TsirigotisJohn Dervenoulas
Sep 27, 2007·Lupus·A W S de SouzaE I Sato
May 23, 2009·Journal of Hypertension·Nicola J RobinsonIan P Crocker
Jan 13, 2010·Lupus·P R J AmesJ Delgado Alves
Jul 6, 2010·Lancet·Eric A P SteegersRobert Pijnenborg
Nov 26, 2010·Obstetrics and Gynecology·Aline D do PradoBernardo L Horta
Dec 23, 2011·Placenta·J L BarthaG Perdomo
Jun 23, 2012·PloS One·Yang BaiYan-ling Wang
Aug 2, 2012·Journal of Obstetrics and Gynaecology of India·J T GohilPriyanka Gupta
Feb 8, 2014·Journal of Reproductive Immunology·Wenda Ramma, Asif Ahmed
Citations
Feb 6, 2017·Archives of Gynecology and Obstetrics·Yonghong WangJun Xia
Sep 22, 2016·Reproductive Sciences·Mareike SandvoßAnibh Martin Das
Apr 1, 2017·Reproductive Sciences·Mareike SandvoßAnibh Martin Das