PMID: 9659605Jul 11, 1998Paper

Effects of serum protein opsonization on cytokine release by titanium-alloy particles

Journal of Biomedical Materials Research
W J MaloneyR Lane Smith

Abstract

This study tested whether macrophages respond differently to retrieved titanium-alloy particles than they do to machined titanium-alloy particles and assessed whether pretreatment of machined titanium-alloy particles with human serum would influence macrophage activation and cytokine release in vitro. Human monocyte/macrophages were isolated from normal healthy donors and exposed to increasing concentrations of machined and retrieved titanium-alloy particles. The profile of cytokine release was determined by commercially available ELISA kits. Machined titanium-alloy particles were opsonized with human serum and added to macrophage cultures. Serum protein binding was confirmed by SDS-PAGE analysis. The results showed that machined titanium-alloy particles and retrieved titanium-alloy particles stimulate a similar level of cytokine release when tested at comparable concentrations. Opsonization of the machined particles with human serum increased the macrophage release of cytokines in the first 12 h after exposure compared to nonopsonized particles. At 24 h, the opsonized particles stimulated significantly higher levels of cytokine release, but only at the greatest particle concentrations. This study demonstrates that machined tit...Continue Reading

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Citations

Aug 30, 2008·HSS Journal : the Musculoskeletal Journal of Hospital for Special Surgery·P Edward PurdueThomas P Sculco
Aug 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yanming BiEdward M Greenfield
Jan 26, 2013·Journal of Biomedical Materials Research. Part a·Sandra C P CachinhoJohn A Hunt
Sep 13, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Keita MiyanishiR Lane Smith
Jan 5, 2002·Journal of Biomedical Materials Research·Toshihiro AkisueYuichi Mochida
Sep 5, 2002·Journal of Biomedical Materials Research·Masatsugu MiyaguchiYoshiki Yamano
May 8, 2003·Journal of Biomedical Materials Research. Part a·Doo-Hoon SunR Lane Smith
May 15, 2003·Journal of Biomedical Materials Research. Part a·Nadim James HallabJoshua J Jacobs
Sep 2, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Diptendu S RakshitP Edward Purdue
Sep 19, 2006·Clinical Orthopaedics and Related Research·P Edward PurdueThomas P Sculco
Dec 4, 2002·Clinical Orthopaedics and Related Research·Thomas W Bauer
Nov 14, 2017·Critical Reviews in Toxicology·Donatella GranchiNicola Baldini

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