Effects of sex-sorted spermatozoa on the efficiency of in vitro fertilization and ultrastructure of in vitro produced bovine blastocysts

Anatomia, histologia, embryologia
G A PalmaF Sinowatz

Abstract

Frozen-thawed sexed semen from six bulls (Holstein) was used for studying their efficiency in an in vitro fertilization (IVF)-programme and to compare their ultrastructure with in vitro produced bovine blastocysts produced with non-sorted sperm. Progressive motility of sorted spermatozoa, their IVF rate, development of produced blastocysts and the ultrastructure of the blastocysts were analysed. The cleavage rates of sexed sperm of bulls (groups S1, S2 and S4) were significantly lower than that of unsorted control sperm (P < 0.01). Blastocyst development at day 7 of the sexed semen groups varied between 3.5% and 28.8% versus 33.6% for non-sexed semen. The individual blastocyst yield with sexed semen of group S5 (28.8%) was similar to the mean blastocyst production of the non-sexed control spermatozoa (C, 33.6%; P > 0.05). The remaining five sexed sperm groups resulted in significantly lower developmental rates of blastocysts on day 7 (S1, 4.9%; S2, 0%; S3, 0%, S4, 3.5%; S6, 25.8%, P < 0.01). Group S2 showed microbiological contamination in 50% (four of eight) and S3 in 100% of the experiments (eight of eight). Progressive motility of sexed sperm was significantly lower than that of unsorted sperm (S1, 48 +/- 12.0%; S2, 41 +/- 1...Continue Reading

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Citations

Oct 5, 2010·Veterinary Medicine International·Giorgio A PresicceFuliang Du
Oct 25, 2008·Theriogenology·J XuF Du
Oct 21, 2016·The Journal of Reproduction and Development·Yasushi InabaTamás Somfai
Mar 15, 2021·Reproduction in Domestic Animals = Zuchthygiene·Ivona TravnickovaMarie Machatkova
Sep 1, 2021·Scientific Reports·Sven ReeseSabine Kölle
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Qifu HeFusheng Quan

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