PMID: 8590784Oct 1, 1995Paper

Effects of short-term culturing on islet phosphoinositide and insulin secretory responses to glucose and carbachol

Acta Diabetologica
W S ZawalichG G Kelley

Abstract

The ability of glucose and carbachol, alone or in combination, to stimulate islet cell phosphoinositide (PI) hydrolysis and insulin secretory responses in freshly isolated or in 20-24 h cultured rat islets was assessed. In freshly isolated, 3H-inositol-prelabeled islets, 20 mM glucose alone or 1 mM carbachol alone stimulated significant increments in 3H-inositol efflux and inositol phosphate (IP) accumulation. When stimulated with both agonists, a dramatic and synergistic effect on IP accumulation was noted. Carbachol (1 mM) alone had no sustained stimulatory effect on insulin secretion. Glucose (20 mM) alone induced a biphasic insulin secretory response. When compared to prestimulatory secretory rates of 18 +/- 4 pg/islet/min, peak first and second phase responses now averaged 422 +/- 61 and 1016 +/- 156 pg/islet/min, respectively. In contrast to freshly studied islets, culturing islets for 20-24 h in CMRL-1066 medium attenuated all measured responses. The increases in 3H-inositol efflux rates in response to glucose, carbachol, or their combination were significantly less than those observed with fresh islets. The IP responses were also attenuated. Second phase insulin secretory responses to 20 mM glucose alone 68 +/- 9 pg/isl...Continue Reading

References

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Citations

Apr 1, 2010·Journal of Nutritional Science and Vitaminology·Hanae YamazakiWalter S Zawalich
Jan 15, 2008·Metabolism: Clinical and Experimental·Walter S Zawalich, Kathleen C Zawalich
Dec 15, 2007·Metabolism: Clinical and Experimental·Walter S ZawalichKathleen C Zawalich
Dec 13, 2006·Metabolism: Clinical and Experimental·Walter S ZawalichHanae Yamazaki
Aug 18, 2005·American Journal of Physiology. Endocrinology and Metabolism·Hanae YamazakiWalter S Zawalich
Jul 27, 2006·American Journal of Physiology. Endocrinology and Metabolism·Hanae YamazakiWalter S Zawalich

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