Effects of Shugan-Jianpi Recipe on the Expression of the p38 MAPK/NF-κB Signaling Pathway in the Hepatocytes of NAFLD Rats

Medicines
Yuanjun DengQinhe Yang

Abstract

Background: In traditional Chinese medicine, the Shugan-Jianpi recipe is often used in the treatment of nonalcoholic fatty liver disease (NAFLD). This study aimed to explore the mechanism of the Shugan-Jianpi recipe in relation to rats with NAFLD induced by a high-fat diet. Methods: Rats were randomly divided into eight groups: normal group (NG), model group (MG), low-dose Chaihu⁻Shugan⁻San group (L-CG), high-dose Chaihu⁻Shugan⁻San group (H-CG), low-dose Shenling⁻Baizhu⁻San group (L-SG), high-dose Shenling⁻Baizhu⁻San group (H-SG), low dose of integrated-recipes group (L-IG), and high dose of integrated-recipes group (H-IG). After 26 weeks, a lipid profile, aspartate, and alanine aminotransferases in serum were detected. The serum levels of inflammatory factors including interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) were analyzed using the enzyme linked immunosorbent assay (ELISA) method. Hepatic pathological changes were observed with hematoxylin-eosin (HE) and oil red O staining. The expression of the p38 mitogen-activated protein kinases (MAPK)/nuclear factor-κB (NF-κB) pathway was detected by quantitative real-time PCR and Western blotting. Results: A pathological section revealed that NAFLD rats have be...Continue Reading

References

Apr 29, 2005·Journal of Leukocyte Biology·Adrian A MaungJames A Lederer
Feb 5, 2011·Nature Reviews. Gastroenterology & Hepatology·Tom Luedde, Robert F Schwabe
Mar 25, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Antero SalminenKai Kaarniranta
Mar 26, 2014·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Etsuko HashimotoJurgen Ludwig
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Jan 30, 2016·Metabolism: Clinical and Experimental·Elena BuzzettiEmmanuel A Tsochatzis
Jul 20, 2017·Nature Immunology·Inna S AfoninaRudi Beyaert
Oct 24, 2017·Toxicon : Official Journal of the International Society on Toxinology·Jean-Marc Cavaillon

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
flow cytometry
PCR
protein assay
electrophoresis
X-ray

Software Mentioned

Statistical Package for the Social Sciences ( SPSS

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