Effects of simulated carbon dioxide and helium peumoperitoneum on proliferation and apoptosis of gastric cancer cells.

World Journal of Gastroenterology : WJG
Ying-Xue HaoPei-Wu Yu

Abstract

To investigate the effects of carbon dioxide (CO(2)) and helium insufflation administered at different pressures on the growth and apoptosis of cultured human gastric cancer cells. The gastric cancer cells MKN-45 were exposed to a CO(2) and helium environment maintained at different pressures (0, 5, 10 and 15 mmHg). The cells were exposed to simulated pneumoperitoneum environment for 4 h, and pH of the culture media was measured after it was moved to normal conditions for 0, 2, 4, 6 and 8 h. Proliferation viability of MKN-45 was examined by 3-[4,5Dimethylthiazol-2-yl],5-diphenyltetrazolium bromide or triazolyl blue (MTT) assay after it was moved to normal conditions. Apoptotic ratio was measured by Annexin V-FITC/PI double labelled staining. The pH of media was acid and recovered to normal after 4 h in the CO(2) group while it was basic in the helium group. There was no difference between CO(2) groups (under 10 mmHg ) and control group (P > 0.05) in the proliferative viability of the cells. The cultured cells exposed to 15 mmHg CO(2) environment grew more slowly than control group from 4 to 7 d (P < 0.01 ) while there was no difference from 1 to 3 d (P > 0.05). The proliferative viability in helium group was not obviously diffe...Continue Reading

References

Aug 1, 1997·Journal of Bioenergetics and Biomembranes·L D ShrodeS Grinstein
Feb 5, 1999·The Australian and New Zealand Journal of Surgery·S J NeuhausD I Watson
Apr 5, 2001·Surgical Endoscopy·R L Whelan
Jul 27, 2001·BJOG : an International Journal of Obstetrics and Gynaecology·F LécuruR Taurelle
Dec 18, 2001·American Journal of Obstetrics and Gynecology·V J SmidtW W Hurd
Sep 17, 2002·Journal of the American College of Surgeons·Paul ZiprinAra W Darzi
Oct 22, 2002·American Journal of Obstetrics and Gynecology·Michael P HopkinsSheri Holda
Jun 12, 2004·American Journal of Surgery·Myriam J Curet
Mar 6, 2007·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Satoru NakagawaHiroshi Yabusaki
Jun 15, 2007·Surgical Oncology·Khalid ShehzadMuhammed Ashraf Memon

❮ Previous
Next ❯

Citations

Dec 3, 2008·Clinical and Experimental Medicine·Ying-Xue HaoBo Tang
Jun 26, 2009·World Journal of Gastroenterology : WJG·Gui-Sen XuYing-Hai Liu
Oct 12, 2011·World Journal of Gastroenterology : WJG·Jun LiFu-Zhou Tian
Feb 4, 2014·Clinics and Research in Hepatology and Gastroenterology·Jiran ZhangDaorong Wang
Dec 23, 2011·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·H-X LuoB Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis