Effects of Simvastatin on the Viability and Secretion of Vascular Endothelial Growth Factor of Cell Spheroids Cultured in Growth Media

Implant Dentistry
Hyunjin LeeJun-Beom Park

Abstract

This study evaluates the effects of simvastatin on the morphology, viability, secretion of vascular endothelial growth factor (VEGF) and expression of stemness markers and messenger RNA of cell spheroids cultured in growth media. Three-dimensional cell spheroids with stem cells and osteoblast-like cells were fabricated using concave, silicon, elastomer-based microwells in the presence of simvastatin at concentrations of 1 and 10 μM. Qualitative cellular viability was determined with a confocal microscope, and quantitative cellular viability was evaluated using a Cell Counting Kit-8 assay. The expression of stem cell surface markers was tested, and a quantitative real-time polymerase chain reaction was performed to evaluate the expression of collagen I and Runx2. The cell spheroids were well formed in the microwells, but the addition of simvastatin produced significant changes in the morphology of spheroids. No significant changes in cellular viability were noted with the addition of simvastatin on day 1, but the addition of simvastatin significantly decreased cellular viability on day 5. The addition of simvastatin significantly increased the secretion of VEGF. The expression of the CD90 surface marker was seen regardless of wh...Continue Reading

References

Feb 13, 2001·Biochemical and Biophysical Research Communications·T MaedaN Horiuchi
Oct 18, 2002·Advanced Drug Delivery Reviews·Cheryl Wong Po Foo, David L Kaplan
Oct 19, 2011·Journal of Biomedicine & Biotechnology·Seogjin Seo, Kun Na
Dec 17, 2014·Tissue & Cell·Hongyang LiJiansu Chen
Apr 16, 2015·PloS One·Dalila Lucíola ZanetteWilson Araújo Silva
May 22, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenzhong HuangWei Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.