Effects of slit divergent ultrasound and enzymatic treatment on the structure and antioxidant activity of arrowhead protein

Ultrasonics Sonochemistry
Chaoting WenHaile Ma

Abstract

The arrowhead has attracted great research interest for their potential applications in pharmacy, food and biomedical areas. However, no information is reported about the nature and structure of the arrowhead protein (AP). Herein, effects of slit divergent ultrasound (28, 33, 40 KHz frequencies at 30-50 °C) and enzymatic (pepsin, trypsin, and alcalase) treatment on structure of AP were studied. In addition, changes in antioxidant activity of AP treated with ultrasound and enzymes were measured by chemical and cellular-based assays. The results showed that ultrasound treatment had considerable impact on the structure of AP and increased the susceptibility of AP to pepsin, trypsin and alcalase proteolysis. The changes in UV-Vis spectra, free sulfhydryl (SH) and disulfide bonds (SS) groups indicated that the structure of AP unfolded after ultrasound treatment. Besides, intrinsic fluorescence intensity of AP was increased by ultrasound treatment and then decreased after following enzymatic treatment. The circular dichroism (CD) analysis showed that ultrasound and enzymatic treatment decreased α-helix, β-turn of content of AP. However, the β-sheet and random coil content of AP increased. Interestingly, the AP after ultrasound and en...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·S S Lehrer, P C Leavis
Feb 15, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·L Góth
Feb 1, 1997·Radiology·P M RaoR A Novelline
Oct 4, 2002·Nutrition·Yun-Zhong FangGuoyao Wu
Aug 11, 2004·Ultrasonics Sonochemistry·Zhong Min TianJu Qing Kang
Feb 3, 2005·Journal of Agricultural and Food Chemistry·Eresha MendisSe-Kwon Kim
Oct 11, 2005·Journal of Proteome Research·D López-FerrerJ Vázquez
Dec 13, 2005·Journal of Environmental Management·Preeti C Sangave, Aniruddha B Pandit
Sep 9, 2006·Ultrasonics Sonochemistry·Ibrahim GülserenJochen Weiss
Jan 15, 2008·Trends in Ecology & Evolution·Hans LambersSally E Smith

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Citations

Apr 6, 2021·Ultrasonics Sonochemistry·Mostafa GoudaXiaoli Li
Jun 19, 2021·Current Research in Food Science·Erwann DurandPierre Villeneuve

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