Effects of small neutral osmolytes on the supercoiling free energy and intrinsic twist of p30delta DNA

Biopolymers
David P RangelJ Michael Schurr

Abstract

Both theory and experiments are employed to investigate the effects of small neutral osmolytes on the average intrinsic twist (l0), the torsion and bending elastic constants, and the twist energy parameter (ET) that governs the supercoiling free energy. The experimental data for ethylene glycol and acetamide at 37 degrees C suggest, and are interpreted in terms of, a model wherein the DNA exhibits an equilibrium between two distinct conformational states that possess different numbers of bound water molecules and exhibit different intrinsic twists and torsion and bending elastic constants. Expressions are derived to relate the effective ET and l0 to the equilibrium constant, water activity (aw), and number (n) of bound water molecules released per cooperative domain undergoing the two-state transition. The variations of l0 and ET with -ln(aw) are similar for acetamide and ethylene glycol at 37 degrees C. Fitting the theory to those data yields the range n = 103-125 for ethylene glycol and n = 71-113 for acetamide, depending on the assumed value of ET for the dehydrated state. The cooperative domain size of the two-state transition is estimated to exceed about 25-30 base pairs (bp). Between 0 and 19.4 w/v % ethylene glycol, the ...Continue Reading

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Citations

Oct 2, 2008·The Journal of Physical Chemistry. B·Greg P BrewoodJ Michael Schurr
Sep 20, 2007·Biopolymers·Fátima Pereira de SouzaJoão Ruggiero Neto
Aug 2, 2005·Biophysical Journal·J Michael SchurrSergio R Aragon
Sep 12, 2006·Biophysical Journal·Bryant S FujimotoJ Michael Schurr
Jul 3, 2008·The Journal of Physical Chemistry. B·Alyssa L SmithBruce H Robinson

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