Effects of sodium citrate, low molecular weight heparin, and prostaglandin E1 on aggregation, fibrinogen binding, and enumeration of equine platelets

American Journal of Veterinary Research
J K KingstonK Jane Wardrop

Abstract

To investigate the effects of sodium citrate, low molecular weight heparin (LMWH), and prostaglandin E1 (PGE1) on aggregation, fibrinogen binding, and enumeration of equine platelets. Blood samples obtained from 4 Thoroughbreds. Blood was collected into syringes in the ratio of 9 parts blood:1 part anticoagulant. Anticoagulants used were sodium citrate, LMWH, sodium citrate and LMWH, or 300 nM PGE1/ml of anticoagulant. Platelet aggregation in response to ADP, collagen, and PGE1 was assessed, using optical aggregometry. Platelet activation was evaluated, using flow cytometry, to detect binding of fluorescein-conjugated anti-human fibrinogen antibody. Plasma concentration of ionized calcium was measured, using an ion-selective electrode. Number of platelets (mean +/- SEM) in samples containing LMWH (109.5+/-11.3 x 10(3) cells/microl) was significantly less than the number in samples containing sodium citrate (187.3+/-30.3 x 10(3) cells/microl). Increasing concentrations of sodium citrate resulted in reductions in platelet aggregation and plasma concentration of ionized calcium. Addition of PGE1 prior to addition of an agonist inhibited platelet aggregation in a concentration-dependent manner, whereas addition of PGE1 4 minutes af...Continue Reading

References

Dec 7, 1979·European Journal of Pharmacology·P Y WongW H Lee
Jul 29, 1976·The New England Journal of Medicine·R B BabcockW B Scharfman
Jan 1, 1976·American Journal of Clinical Pathology·P H Levine
Jan 1, 1986·Haemostasis·J G Kelton
Apr 1, 1985·Seminars in Thrombosis and Hemostasis·L D Brace, J Fareed
Feb 10, 1983·The New England Journal of Medicine·E L Schneider
Dec 1, 1994·British Journal of Haematology·K E GoadH R Gralnick
Sep 1, 1996·Transfusion Medicine·R J HawkerS G Mitchell

❮ Previous
Next ❯

Citations

Jan 25, 2003·Veterinary Immunology and Immunopathology·Cory C LalkoBenjamin J Darien
Jul 1, 2008·Medical Devices : Evidence and Research·Cristina SolomonNiels Rahe-Meyer
May 1, 2013·American Journal of Veterinary Research·April E BlongBenjamin M Brainard
Jan 9, 2004·Journal of the American Veterinary Medical Association·M Kimberley J McGurrinDorothee Bienzle
Oct 19, 2010·Veterinary Medicine International·Alessia GiordanoSaverio Paltrinieri
Jan 21, 2016·Veterinary and Comparative Orthopaedics and Traumatology : V.C.O.T·Juan C GonzálezJorge U Carmona
Feb 18, 2016·International Journal of Dentistry·Hooman KhorshidiHodasadat Banihashemi
Jul 26, 2008·The Veterinary Clinics of North America. Equine Practice·Marjory B Brooks
Oct 27, 2004·The Veterinary Journal·R Mischke, U Schulze
Sep 14, 2006·Research in Veterinary Science·D ArgüellesM Prades
Jan 8, 2008·Journal of Surgical Oncology·Marcus LangenskiöldMarie-Louise Ivarsson
Jun 13, 2020·Journal of Thrombosis and Thrombolysis·Diana A Gorog, Richard C Becker
Dec 8, 2017·Veterinary Medicine International·Claudia M GutiérrezJorge U Carmona

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Veterinary Internal Medicine
Elizabeth G DavisBonnie R Rush
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
William J Lindblad
Journal of Veterinary Emergency and Critical Care
Kira L EpsteinJames N Moore
© 2021 Meta ULC. All rights reserved