Abstract
This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the proliferation, odontoblastic differentiation, and angiogenic potential of human dental pulp cells (HDPCs). Differentiation was measured by alkaline phosphatase activity, calcified nodule formation by alizarin red staining, and marker messenger RNA (mRNA) levels by reverse-transcription polymerase chain reaction. In vitro angiogenesis was quantified by migration, mRNA levels of angiogenic genes, and endothelial tube formation. STP and SHMP dose dependently increased the proliferation and ALP activity and enhanced mineralized nodule formation and odontoblast marker mRNAs of HDPCs. STP and SHMP resulted in the up-regulation of angiogenic genes in HDPCs. Endothelial cells treated with conditioned medium collected from STP- and SHMP-exposed HDPCs showed an increase in migration and capillary tube formation. Knockdown of the expression of the genes encoding of inorganic pyrophosphate by small interfering RNA attenuated the STP- and SHMP-indu...Continue Reading
References
Mar 17, 1995·The Journal of Biological Chemistry·K D Kumble, A Kornberg
May 13, 2003·The Journal of Biological Chemistry·Toshikazu ShibaMinako Ohshiro
Jul 24, 2004·Journal of Dental Research·K IoharaA Akamine
Jul 24, 2004·Journal of Dental Research·Y KawazoeT Kohgo
Jan 12, 2005·Journal of Clinical Periodontology·Suru MankodiTao He
Apr 19, 2005·Genes & Development·Monzur MurshedGerard Karsenty
May 28, 2005·The American Journal of Pathology·H Clarke AndersonJosé Luis Millán
Feb 20, 2007·Oral Diseases·A J Sloan, A J Smith
Mar 14, 2007·Archives of Oral Biology·Masae KitagawaTakashi Takata
Mar 27, 2007·The Journal of Biological Chemistry·William N AddisonMarc D McKee
Aug 28, 2007·Journal of Dental Research·Y HacchouK Furusawa
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrey Y AbramovEvgeny Pavlov
Feb 16, 2008·International Journal of Biological Sciences·Yumi KawazoeToshikazu Shiba
Aug 30, 2008·Cells, Tissues, Organs·Thorsten KirschJeffrey A Winkles
Mar 3, 2009·Journal of Endodontics·Li WangBin Peng
Feb 11, 2010·Journal of Bone and Mineral Metabolism·Daiki MorimotoHideki Yoshikawa
Feb 23, 2010·Journal of Endodontics·Kyung-San MinEun-Cheol Kim
Mar 25, 2010·Journal of Dental Research·Y UsuiK Furusawa
Jul 22, 2010·Journal of Endodontics·Sun-Ju KimEun-Cheol Kim
Oct 19, 2010·Journal of Endodontics·Young-Suk KimEun-Cheol Kim
Nov 26, 2010·Antimicrobial Agents and Chemotherapy·Ji-Hoi MoonJin-Yong Lee
Mar 23, 2011·Stem Cell Research·Adam OskowitzRadhika Pochampally
Sep 21, 2011·The Journal of Biological Chemistry·Hwa Kyung NamNan E Hatch
Jan 25, 2012·Journal of Dental Research·S-Y LeeE-C Kim
May 19, 2012·Journal of Endodontics·Eun-Cheol KimYoungho Kim
Jun 19, 2012·Journal of Endodontics·Jong-Jin KimEun-Cheol Kim
Aug 21, 2013·PloS One·Annelies BronckaersIvo Lambrichts