Effects of solvent viscosity on ligand interconversion dynamics in the primary docking site of heme proteins

Journal of the American Chemical Society
Seongheun KimManho Lim

Abstract

Interconversion dynamics of the ligand in the primary docking site of myoglobin (Mb) and hemoglobin (Hb) in trehalose and glycerol/D2O mixtures at 283 K was investigated by probing time-resolved vibrational spectra of CO photolyzed from these proteins. The interconversion dynamics in viscous media are similar to those in aqueous solution, indicating that it is minimally coupled to the solvent-coupled large-scale protein motion. Interconversion rates in the heme pocket of Hb in water solution are slower than those of Mb in trehalose glass, suggesting that the interconversion barrier in Hb is intrinsically higher than that in Mb and is not modified by the solvent viscosity.

References

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Citations

Jan 28, 2016·Physical Chemistry Chemical Physics : PCCP·Taegon LeeManho Lim
Aug 15, 2015·Physical Chemistry Chemical Physics : PCCP·Cheolhee YangHyotcherl Ihee

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