Effects of Somatostatin Analogs and Dopamine Agonists on Insulin-Like Growth Factor 2-Induced Insulin Receptor Isoform A Activation by Gastroenteropancreatic Neuroendocrine Tumor Cells

Neuroendocrinology
Roxanne C S van AdrichemLeo J Hofland

Abstract

Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) express insulin-like growth factor (IGF)-related factors [IGF1, IGF2; insulin receptor (IR)-A, IR-B; IGF-binding protein (IGFBP) 1-3] as well as somatostatin (SSTRs) and dopamine D2 receptors (D2Rs). To (1) compare mRNA expression of IGF-related factors in human pancreatic NET (panNET) cell lines with that in human GEP-NETs to evaluate the usefulness of these cells as a model for studying the IGF system in GEP-NETs, (2) determine whether panNET cells produce growth factors that activate IR-A, and (3) investigate whether somatostatin analogs (SSAs) and/or dopamine agonists (DAs) influence the production of these growth factors. In panNET cells (BON-1 and QGP-1) and GEP-NETs, mRNA expression of IGF-related factors was measured by quantitative real-time PCR. Effects of the SSAs octreotide and pasireotide (PAS), the DA cabergoline (CAB), and the dopastatin BIM-23A760 (all 100 nM) were evaluated at the IGF2 mRNA and protein level (by ELISA) and regarding IR-A bioactivity (by kinase receptor activation assay) in panNET cells. panNET cells and GEP-NETs had comparable expression profiles of IGF-related factors. Especially in BON-1 cells, IGF2 and IR-A were most highly expressed. P...Continue Reading

Citations

Apr 13, 2018·Endocrine-related Cancer·Giovanni VitaleAnnamaria Colao
Oct 27, 2017·Expert Opinion on Pharmacotherapy·Gherardo MazziottiAndrea Giustina
Sep 29, 2018·The Journal of Clinical Endocrinology and Metabolism·Aura D Herrera-MartínezRaúl M Luque
Oct 12, 2018·Endocrine-related Cancer·Thomas CunyLeo J Hofland
Oct 4, 2017·Endocrine Reviews·Antonino BelfioreRiccardo Vigneri
Feb 5, 2021·Journal of Clinical Medicine·Maria Celeste CantoneGiovanni Vitale

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