PMID: 9557962Apr 29, 1998Paper

Effects of some antigenic fractions of Leishmania major on nitric oxide production and IL-12 secretion by murine peritoneal macrophages

Immunology Letters
S MirshahidiT Imir

Abstract

Nitric oxide (NO) and IL-12 are important mediators of the immune response to Leishmania major. In this study, the effects of L. major promastigotes, crude antigenic fraction (CAF) and its subfractions on NO production and IL-12 secretion by BALB/c mice peritoneal macrophages is investigated. The subfractions of CAF, namely, fractions 1, 2 and 3, that were in the molecular weight range of 97.4-66, 66-45 and below 45 kDa, respectively, were separated by SDS-PAGE. NO production was determined by using Griess reagent and IL-12 was measured by ELISA. It was found that NO production was stimulated by promastigotes but not by CAF or its subfractions. IL-12 secretion was stimulated by promastigotes, CAF and fraction 1 while fractions 2 and 3 did not have any effect.

References

Mar 1, 1990·The Journal of Clinical Investigation·S G ReedK H Grabstein
Nov 30, 1988·Biochemical and Biophysical Research Communications·J B HibbsE M Rachlin
May 1, 1994·Immunology Letters·L Q VieiraP Scott
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Aug 1, 1997·Immunology Today·J E Allen, R M Maizels

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Citations

May 17, 2011·Mutation Research·Larissa Ragozo Cardoso de OliveiraSueli Aparecida Calvi
Oct 24, 2007·Microbiology and Immunology·Mohammad Hossein AlimohammadianAlireza Mofarrah

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