PMID: 9421186Jan 8, 1998Paper

Effects of sphingomyelin and phosphatidylcholine degradation on cyclodextrin-mediated cholesterol efflux in cultured fibroblasts

Biochimica Et Biophysica Acta
H OhvoJ P Slotte

Abstract

The hydrolysis of plasma membrane sphingomyelin is known to dramatically alter cellular cholesterol homeostasis in different ways, whereas the degradation of plasma membrane phosphatidylcholine has much less or no effects on cell cholesterol homeostasis [Pörn, Ares, Slotte, J. Lipid Res. 34 (1993) 1385-1392]. In this study, we used an efficient extracellular cholesterol acceptor (cyclodextrin) and determined the extent of cholesterol efflux from cultured fibroblasts in which plasma membrane sphingomyelin or phosphatidylcholine was degraded. Treatment of cells with sphingomyelinase reduced the cell sphingomyelin content by about 76% (about 13 nmol SM degraded), and dramatically increased the desorption of [3H]cholesterol from the plasma membrane to 2-hydroxypropyl-beta-cyclodextrin. The corresponding hydrolysis of cell surface phosphatidylcholine (about 12% reduction of the cellular phosphatidylcholine content, corresponding to about 12 nmol degraded PC) had almost no effect on cell [3H]cholesterol efflux. The stimulatory effect of sphingomyelin degradation on cell [3H]cholesterol efflux was reversible, since rates of [3H]cholesterol efflux dropped back to control levels when cells (in this case baby hamster kidney cells) were a...Continue Reading

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Citations

Sep 23, 2000·Chemistry and Physics of Lipids·J P Slotte
Mar 23, 2002·The Journal of Biological Chemistry·Peter MattjusRhoderick E Brown
Jul 26, 2002·Seminars in Cell & Developmental Biology·Will Prinz
Sep 17, 2005·Archives of Biochemistry and Biophysics·Roumen PankovAlbena Momchilova
Jul 16, 2005·Journal of Colloid and Interface Science·Andrew J GuarinoSteven P Wrenn
May 26, 2015·Biochimica Et Biophysica Acta·Daniel Wüstner, Katarzyna Solanko
Jul 21, 2010·Biophysical Journal·Beate BoulgaropoulosGeorg Pabst
Mar 3, 2004·Biophysical Journal·Juha M HolopainenPaavo K J Kinnunen
Dec 11, 2007·Biochimica Et Biophysica Acta·Mojca Podlesnik BesenicarGregor Anderluh
Mar 1, 2003·Biophysical Journal·Jared C LawrenceRobert M Henderson
Aug 30, 2005·Biochimica Et Biophysica Acta·Sonja M K AlankoBodil Ramstedt
Jan 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Michael Edidin
May 7, 1999·Molecular Reproduction and Development·N L Cross
May 3, 2005·Journal of Lipid Research·Katharina GausWendy Jessup
Sep 16, 2005·Journal of Lipid Research·Papasani V SubbaiahKewei Wang
Nov 8, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Guosong Qiu, John S Hill
Nov 14, 2013·The Journal of Biological Chemistry·Kailash GulshanJonathan D Smith
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Shuhui WangKailash Gulshan
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandre K Rouquette-JazdanianClaude Aussel
Aug 5, 2009·Chemistry and Physics of Lipids·Karin KlappeJan Willem Kok

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