Effects of spinal nerve ligation on immunohistochemically identified neurons in the L4 and L5 dorsal root ganglia of the rat

The Journal of Comparative Neurology
Donna L HammondBrian Elzey

Abstract

This study examined the effect of spinal nerve ligation on different populations of immunohistochemically identified neurons in the dorsal root ganglia (DRG) of the rat. The optical fractionator method was used to count neurons in the ipsilateral L4 and L5 DRG 1-20 weeks after ligation of the L5 and L6 spinal nerves, sham surgery, or no surgery. One week after ligation, neurons in the L5 DRG that were labeled by IB4, a marker of unmyelinated primary afferent neurons, were largely absent. The numbers of IB4-labeled neurons then progressively increased to reach control values by 20 weeks. A smaller, sustained decrease occurred in the number of small-, medium- and large-sized neurons immunoreactive for calcitonin gene-related peptide (CGRP), a marker for peptidergic primary afferents, in the L5 DRG. There was a proportionately greater decrease in the numbers of medium- to large-sized CGRP-like immunoreactive neurons. The number of myelinated afferents in the L5 DRG, identified by their staining for neurofilament protein (N52), did not change after ligation. However, closer examination revealed a significant decrease in the numbers of large-sized neurons, coupled with an increase in the numbers of small- to medium-sized neurons, an...Continue Reading

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Citations

Jun 16, 2011·Histochemistry and Cell Biology·Adam J ReidMikael Wiberg
Sep 1, 2012·Journal of Neurotrauma·Daniel J ChewPeter J Shortland
Dec 24, 2005·Journal of Dental Research·R Wadachi, K M Hargreaves
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Apr 30, 2013·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Tetsuhiro IshikawaSeiji Ohtori
Dec 18, 2009·The Journal of Pain : Official Journal of the American Pain Society·Daniel VilceanuCheryl L Stucky
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Jan 20, 2006·The European Journal of Neuroscience·Peter J ShortlandSharon Averill
May 28, 2011·The Journal of Comparative Neurology·Bruce MesnageMatilde Cordero-Erausquin
Nov 3, 2009·The Journal of Comparative Neurology·Sanja Lovric KojundzicDamir Sapunar
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Feb 24, 2015·Restorative Neurology and Neuroscience·Laura Casals-DíazXavier Navarro
Nov 8, 2011·Pain·Julien PelletierDominique Sigaudo-Roussel

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