Effects of sporidesmin on cultured biliary tract cells from Romney lambs that differed in their sensitivity to sporidesmin

New Zealand Veterinary Journal
G C LindsayT W Jordan

Abstract

AIMS To investigate the effects of sporidesmin on cells cultured from the epithelial surface of sheep gallbladder walls, and to examine cellular responses to sporidesmin using cultures prepared from the gallbladders of sheep from selection lines that differed in sensitivity to sporidesmin-induced liver damage. METHODS Gallbladders were obtained following slaughter of lambs that were selected for resistance or susceptibility to sporidesmin-induced liver damage, or were not selected (controls). Monolayer cell cultures were established after incubation of excised gallbladders with protease to detach the lining epithelial cells from the muscular and connective tissue of the gallbladder wall. Released cells were harvested and transferred to culture flasks or dishes, then incubated with 1 µg/mL sporidesmin and were examined at 5 minute intervals, up to 3 hours, using light microscopy to monitor loss of attachment of cultured cells. Immunofluorescence staining of cell cultures was used to identify cytokeratin 19 as a marker for biliary epithelial cells, and to characterise sporidesmin-induced change in the cellular distribution of actin microfilaments. Gallbladder size was also measured. RESULTS In cultures incubated with sporidesmin,...Continue Reading

References

Jan 1, 1990·Journal of Ocular Pharmacology·H K SoongB Lee
Jul 30, 1974·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·G BullockT F Slater
May 1, 1974·The Journal of Cell Biology·A F MirandaS W Tanenbaum
Oct 1, 1968·Journal of Comparative Pathology·P H Mortimer, T A Stanbridge
Apr 1, 1969·Journal of Comparative Pathology·P H Mortimer, T A Stanbridge
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A Müllbacher, R D Eichner
Apr 15, 1983·The Biochemical Journal·S J Cordiner, T W Jordan
Jul 21, 2005·New Zealand Veterinary Journal·B L Smith
Dec 31, 2008·Journal of Experimental & Clinical Cancer Research : CR·Leticia Oliveira-FerrerGunter Schuch

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Citations

Feb 15, 2020·New Zealand Veterinary Journal·T W Jordan
May 1, 2021·Animals : an Open Access Journal From MDPI·Miguel FernándezM Carmen Ferreras

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Methods Mentioned

BETA
light microscopy

Software Mentioned

R Development Core Team
DP Controller
R Foundation for Statistical Computing
R

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