Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.

Applied and Environmental Microbiology
Arturo Ramirez-PeraltaPeter Setlow

Abstract

Bacillus subtilis spores prepared in rich medium germinated faster with nutrient germinants than poor-medium spores as populations in liquid and multiple individual spores on a microscope slide. Poor-medium spores had longer average lag times between mixing of spores with nutrient germinants and initiation of Ca-dipicolinic acid (CaDPA) release. Rich-medium spores made at 37°C germinated slightly faster with nutrient germinants than 23°C spores in liquid, but not when spores germinated on a slide. The difference in germination characteristics of these spore populations in liquid was paralleled by changes in expression levels of a transcriptional lacZ fusion to the gerA operon, encoding a germinant receptor (GR). Levels of GR subunits were 3- to 8-fold lower in poor-medium spores than rich-medium spores and 1.6- to 2-fold lower in 23°C spores than 37°C spores, and levels of the auxiliary germination protein GerD were 3.5- to 4-fold lower in poor medium and 23°C spores. In contrast, levels of another likely germination protein, SpoVAD, were similar in all these spores. These different spores germinated similarly with CaDPA, and poor-medium and 23°C spores germinated faster than rich-medium and 37°C spores, respectively, with dode...Continue Reading

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Citations

Feb 19, 2013·Journal of Bacteriology·Einat SegevSigal Ben-Yehuda
Feb 22, 2012·Journal of Bacteriology·Sonali GhoshPeter Setlow
Apr 30, 2013·Journal of Bacteriology·Arturo Ramirez-PeraltaPeter Setlow
Apr 30, 2013·Journal of Bacteriology·Srishti GuptaGraham Christie
Aug 21, 2012·Journal of Bacteriology·Xuan Yi ButzinPeter Setlow
Jan 22, 2013·Journal of Bacteriology·George Korza, Peter Setlow
Apr 24, 2014·BMC Microbiology·Elisabeth H MadslienToril Lindbäck
Feb 11, 2015·Molecular Cell·Lior SinaiSigal Ben-Yehuda
Mar 20, 2014·Journal of Applied Microbiology·J M CraneG C Bergstrom
Mar 3, 2015·International Journal of Food Microbiology·Alicja K WardaMasja N Nierop Groot
Dec 10, 2013·Applied and Environmental Microbiology·Katja NaglerRalf Moeller
May 8, 2019·Journal of Bacteriology·Emily CamilleriPeter Setlow
Jul 13, 2017·Annual Review of Microbiology·Peter SetlowYong-Qing Li
Nov 1, 2013·Comprehensive Reviews in Food Science and Food Safety·Rocío I Corona-HernandezAbraham Wall-Medrano
Mar 31, 2019·Anaerobe·Ritu Shrestha, Joseph A Sorg
Jun 20, 2021·Comprehensive Reviews in Food Science and Food Safety·Alessia I DelbrückAlexander Mathys

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