Effects of STC1 overexpression on tumorigenicity and metabolism of hepatocellular carcinoma

Oncotarget
Cherry Ct Leung, Chris Kc Wong

Abstract

Stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. Using clinicopathological data, we previously reported that a greater expression of STC1 in hepatocellular carcinoma (HCC) was significantly correlated with smaller tumor size. The underlying mechanism on the correlation is not known. In this study, using a metastatic HCC cell-line (MHCC-97L, P) and lentiviral vector mediated-STC1 overexpression, the inoculation of STC1-overexpressing MHCC-97L (S1) cells in a nude mice xenograft model demonstrated reductions in tumor mass and volume. As compared with P cells, S1 cells exhibited epithelial phenotype with significantly lower plating efficiency and reduced migratory and proliferative potential. Using coulter counter for cell-sizing, S1 cells (17.6 μm) were significantly smaller than P cells (19.6 μm). Western blot analysis revealed that S1 cells exhibited reduced expression level of phosphorylated ribosomal protein S6 (p-rpS6). Moreover, an inhibition of the upstream kinase p70S6Kwas evident with the dephosphorylation of Thr389 in the linker domain of the kinase. The inhibition of p70S6K/p-rpS6 pathway was accompanied with reduced cellular ATP level and increase of p-AMPK in S1 cells. Sig...Continue Reading

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Citations

May 30, 2020·Journal of Cellular and Molecular Medicine·Fangyu ZhaoYupei Zhao
Aug 23, 2019·Molecular Medicine Reports·Yan Xiong, Qibai Wang
Jun 27, 2020·Cancers·Aishani SachdevaIrene Chong
Oct 14, 2019·General and Comparative Endocrinology·Kaidian YangHuiming Ju
Oct 20, 2020·Translational Oncology·Cherry C T Leung, Chris K C Wong

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Methods Mentioned

BETA
transmission
transgenic
xenograft
xenografts
PCR
flow cytometry
light microscopy
Protein Assay

Software Mentioned

SigmaStat
StepOne
Minitab
ImageJ

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