Effects of stimulating interleukin -2/anti- interleukin -2 antibody complexes on renal cell carcinoma

BMC Urology
Kyu-Hyun HanJaeseok Yang

Abstract

Current therapies for advanced renal cell carcinoma (RCC) have low cure rates or significant side effects. It has been reported that complexes composed of interleukin (IL)-2 and stimulating anti-IL-2 antibody (IL-2C) suppress malignant melanoma growth. We investigated whether it could have similar effects on RCC. A syngeneic RCC model was established by subcutaneously injecting RENCA cells into BALB/c mice, which were administered IL-2C or phosphate-buffered saline every other day for 4 weeks. RCC size was measured serially, and its weight was assessed 4 weeks after RENCA injection. Immune cell infiltration into RCC lesions and spleen was assessed by flow cytometry and immunohistochemistry. IL-2C treatment increased the numbers of CD8(+) memory T and natural killer (NK) cells in healthy BALB/c mice (P < 0.01). In the spleen of RCC mice, IL-2C treatment also increased the number of CD8(+) memory T, NK cells, and macrophages as compared to PBS-treated controls (P < 0.01). The number of interferon-γ- and IL-10-producing splenocytes increased and decreased, respectively after 4 weeks in the IL-2C-treated mice (P < 0.01). Tumor-infiltrating immune cells including CD4(+) T, CD8(+) T, NK cells as well as macrophages were increased in ...Continue Reading

References

Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R A FiglinA S Belldegrun
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christopher A KlebanoffNicholas P Restifo
Apr 13, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael B Atkins
Jun 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mark P RubinsteinJonathan Sprent
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sven MostböckHelen Sabzevari
May 20, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bernard EscudierRonald M Bukowski
Oct 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jakub TomalaMarek Kovar
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carsten KriegOnur Boyman
Apr 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Corey SmithRajiv Khanna
Jun 5, 2012·The New England Journal of Medicine·Suzanne L TopalianMario Sznol
Oct 10, 2014·Cancer Research·Yosra MessaiSalem Chouaib
May 15, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Naomi B HaasRobert S DiPaola

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Citations

Jan 25, 2019·NPJ Vaccines·Xuan-Yi WangYu-Mei Wen

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS

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