Effects of storage and thawing conditions on coagulation testing

International Journal of Laboratory Hematology
Robert C GosselinDenis W Dwyre

Abstract

Current recommendations for coagulation testing storage and thawing are based on historical studies that were performed using unbuffered 3.8% sodium citrate. We sought to measure the effects of freezing and thawing conditions 3.2% buffered sodium citrate plasma samples that have been stored in vials with either snap or sealed screw tops, frozen in -70 °C freezer or dry ice and thawed either capped or uncapped. Shed blood samples were pooled and then aliquoted into four snap top and four screw tops vials. Half the vials were stored in a -70 °C freezer, and half on dry ice for at least 16 h. Afterwards, half the frozen samples were thawed in 37 °C waterbath capped, and other half were thawed capped. After thawing cycles, samples were tested for PT, activated partial thromboplastin time (APTT), fibrinogen, D-dimer, factor assays, von Willebrand factor activity, plasminogen, antithrombin, protein C and lupus anticoagulant. Prothrombin time, APTT, factor X, and lupus anticoagulant testing were affected by all vials, freezing and thawing conditions, whereas fibrinogen, D-dimer, von Willebrand activity or protein C were not affected by any vial, freezing or storage condition. Storage vials, freezing and thawing condition affect coagul...Continue Reading

References

Nov 18, 1998·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·D AdcockR A Marlar
Aug 26, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·L V RaoM T Elghetany
Jul 19, 2001·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·B WoodhamsY Gourmelin
Sep 18, 2012·International Journal of Laboratory Hematology·I MackieUNKNOWN British Committee for Standards in Haematology

❮ Previous
Next ❯

Citations

Sep 11, 2016·Clinical Biochemistry·Dorothy M AdcockGiuseppe Lippi
Mar 15, 2019·Current Pharmaceutical Biotechnology·Moutinho BeatrizBotelho Monica
Aug 28, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Lene TrondsetåsIngrid Alsos Lian
Sep 25, 2019·International Journal of Laboratory Hematology·Ann Helen KristoffersenKristin Moberg Aakre
Jun 26, 2015·International Journal of Laboratory Hematology·A McFarlaneR Padmore
Dec 29, 2020·International Journal of Laboratory Hematology·Su-Yeon JoHyerim Kim
Jul 22, 2021·International Journal of Laboratory Hematology·Robert C Gosselin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.