Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples

Journal of Clinical Microbiology
H H KesslerR E Stauber

Abstract

In this study, we compared serum hepatitis C virus (HCV) RNA concentrations with HCV RNA concentrations in whole blood collection tubes, including two different types of EDTA tubes and nucleic acid stabilization tubes (NASTs). We also investigated the impact of a processing delay on HCV RNA concentration in these tubes. In NASTs, the mean HCV RNA concentration was comparable to the mean serum HCV RNA concentration at "date zero." In EDTA tubes, mean baseline HCV RNA concentrations were higher. Storage at room temperature up to 96 h did not result in a decline of HCV RNA concentration in any of the whole blood collection tubes. In NASTs, HCV RNA concentrations remained stable during the whole study period, whereas a significant increase of HCV RNA was observed in both types of EDTA tubes at 96 h compared to date zero. We concluded that HCV RNA remains stable in NASTs at room temperature for at least 96 h, allowing greater flexibility in sample collection and transport.

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Citations

Mar 8, 2003·Biologicals : Journal of the International Association of Biological Standardization·Marta JoséJuan I Jorquera
Nov 5, 2002·Clinical and Diagnostic Laboratory Immunology·Harald H KesslerRudolf E Stauber
Oct 12, 2012·Journal of Clinical Microbiology·Mohammad R HasanPeter Tilley
Nov 28, 2002·Journal of Clinical Microbiology·A R BergmannE Aberer
Jan 1, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Paolo GravinaSergio Bernardini
Aug 14, 2010·Forensic Science International : Synergy·Sara PartemiAntonio Oliva
Mar 1, 2007·Forensic Science International. Genetics·M Bauer
Oct 21, 2009·Journal of Viral Hepatitis·B PutkaM Merheb
Jun 3, 2004·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·G GessoniG Marchiori
Oct 9, 2002·BMC Infectious Diseases·Anne-Isabelle de Moreau de GerbehayePatrick Goubau

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