Effects of storage conditions and duration on cobalamin concentration in serum samples from cats and dogs

Journal of the American Veterinary Medical Association
Jennifer KempfPeter H Kook

Abstract

OBJECTIVE To evaluate the effects of storage conditions and duration on cobalamin concentration in serum samples from dogs and cats. DESIGN Experiment. SAMPLE Serum samples from 9 client-owned cats and 9 client-owned dogs. PROCEDURES Serum harvested from freshly obtained blood samples was separated into 11 aliquots/animal. One aliquot (baseline sample) was routinely transported in light-protected tubes to the laboratory for cobalamin assay; each of the remaining aliquots was stored in a refrigerator (6°C; n = 5) or at room temperature (20°C) with exposure to daylight (5) for 24, 48, 72, 96, or 120 hours. Aliquots were subsequently wrapped in aluminum foil, frozen (-20°C), and then transported to the laboratory for measurement of cobalamin concentration, all in the same run. Percentage decrease in cobalamin concentration from baseline was analyzed by means of linear mixed modeling. RESULTS No differences in cobalamin values were identified between cats and dogs; therefore, data for both species were analyzed together. Median baseline serum cobalamin concentration was 424 ng/L (range, 178 to 1,880 ng/L). Values for serum samples stored with daylight exposure at room temperature were significantly lower over time than were values ...Continue Reading

References

May 9, 2009·Journal of Veterinary Internal Medicine·C G RuauxD A Williams
Nov 3, 2012·Schweizer Archiv für Tierheilkunde·P H KookC E Reusch
Dec 29, 2012·American Journal of Veterinary Research·Nora BerghoffJörg M Steiner
Jul 24, 2013·Journal of Veterinary Internal Medicine·P WorhunskyK W Simpson
Dec 10, 2015·Journal of Veterinary Internal Medicine·L ToressonT Spillmann
Mar 5, 2016·Journal of Veterinary Internal Medicine·B M GeesamanK R Viviano

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Citations

Nov 24, 2019·Journal of Veterinary Internal Medicine·Stefanie KatherRomy M Heilmann

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