Effects of storage conditions on lymphocyte phenotypes from healthy and diseased persons

Journal of Clinical Laboratory Analysis
C H Miller, N B Levy

Abstract

Anticoagulant and room temperature storage of peripheral blood was evaluated in healthy and diseased persons (AIDS and CLL) for its effect upon lymphocyte phenotyping by flow cytometry. Whole blood was stored in either Li heparin, EDTA, or ACD for up to 96 hours. Aliquots at 5 time intervals were evaluated using CD3, CD2, CD4, CD8, and CD19 monoclonal antibodies. Blood from healthy donors stored in EDTA produced an apparent increase in T cells and decrease in B cells most evident by 96 hours storage. Blood from diseased persons stored in Li heparin up to 96 hours showed insufficient changes in phenotype to warrant a different clinical interpretation. We conclude that all three anticoagulants are adequate for short term (less than 24 hr) room temperature transport and storage of peripheral blood for flow cytometry. Li heparin or ACD are more appropriate if prolonged transport times are anticipated.

References

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Citations

Sep 27, 1993·Journal of Immunological Methods·J K Nicholson, T A Green
Nov 14, 1998·Disease-a-month : DM·H W HorowitzG P Wormser
Jan 1, 1990·Journal of Neurotrauma·K B QuattrocchiF C Wagner
Feb 2, 2006·Journal of the National Cancer Institute·Sichuan XiJennifer Rubin Grandis
Aug 8, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrea TompaMaria Faresjö
Aug 15, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·W K HootsC Contant
Mar 1, 1992·The Journal of Urology·C H MillerA R Stone
Feb 11, 2021·Molecular Biology Reports·Yanru XingWen-Jing Wang

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