Effects of Streptococcus mutans gtfC deficiency on mixed oral biofilms in vitro

Caries Research
Thomas ThurnheerB Guggenheim

Abstract

The aim of this study was to examine the influence of glucosyltransferase-gene-negative (gtf-) Streptococcus mutans strains unable to synthesize water-insoluble or soluble glucan on the structure and macromolecular diffusion properties of in vitro grown mixed oral biofilms. Biofilms modeling supragingival plaque consisted of Actinomyces naeslundii OMZ 745, Candida albicans OMZ 110, Fusobacterium nucleatum KP-F2, Streptococcus oralis SK 248, Veillonella dispar ATCC 17748T and one of the S. mutans strains UA159, OMZ 966, OMZ 937 or OMZ 977. Biofilms were grown anaerobically on sintered hydroxyapatite disks for 64.5 h at 37 degrees C. To perform confocal laser scanning microscopy analyses, microorganisms were stained with Syto 13 and extracellular polysaccharides (EPS) with Calcofluor. Macromolecular diffusion properties were measured following timed biofilm exposure to Texas-Red-labeled 70-kDa dextran. Results showed that replacing wild-type S. mutans by a gtfC- mutant led to an increase in the volume fraction occupied by cells from 29 to 48% and a decrease of the EPS volume fraction from 51 to 33%. No such changes were observed when the S. mutans wild-type strain was replaced by a gtfB- or gtfD- mutant. The diffusion coefficient...Continue Reading

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Citations

Sep 26, 2006·Odontology·Jacob M ten Cate
Oct 9, 2007·Journal of Bacteriology·Sang-Joon AhnRobert A Burne
Dec 10, 2013·Clinical Oral Investigations·Thomas ThurnheerPatrick R Schmidlin
Nov 3, 2011·International Journal of Dentistry·Eva-Maria DeckerChristiane von Ohle
Mar 17, 2010·Expert Opinion on Therapeutic Patents·Fu Chen, Dong Wang
Jan 7, 2016·Frontiers in Microbiology·Lei LeiTao Hu
Dec 9, 2014·Virulence·Thomas Thurnheer, Georgios N Belibasakis
Jun 20, 2014·Clinical Oral Implants Research·Alexis IoannidisPatrick R Schmidlin
Jun 1, 2016·Microbiological Research·Jianying ZhangMin Liang
Apr 7, 2017·Molecular Oral Microbiology·Susanne BlochChristina Schäffer
Jun 9, 2007·Microscopy Research and Technique·Steven Reese, Bernhard Guggenheim
Sep 5, 2006·Acta Radiologica·R JunikK Debska-Kozinska
Jun 3, 2015·Molecular Oral Microbiology·T ThurnheerG N Belibasakis
Jul 21, 2017·Caries Research·Sebastian SchlaferViduthalai R Regina
Feb 23, 2020·BioMed Research International·Wei QiuBiao Ren
Nov 22, 2012·Clinical Implant Dentistry and Related Research·Philipp SahrmannThomas Thurnheer
May 13, 2021·International Journal of Clinical Pediatric Dentistry·Srihari Nirguna ChandrasekharHenna P Salim
Apr 30, 2014·Journal of Agricultural and Food Chemistry·Irene Muñoz-GonzálezM Victoria Moreno-Arribas

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