Effects of substrate, passage rate, and pH in continuous culture on flows of conjugated linoleic acid and trans C18:1

Journal of Dairy Science
X QiuJ L Firkins

Abstract

A dual-flow continuous culture system consisting of 4 fermenters was used in a 4 x4 Latin square design. The objective of the research was to evaluate the effects of solid dilution rate (SDR), pH, and concentration of linoleic acid (LA) in the feed mixture on the production of conjugated linoleic acid (CLA) and trans-C18:1. The 4 treatments were 1) control = pH 6.5, 1% LA, 4%/h SDR; 2) high solid dilution rate (HSDR) = pH 6.5, 1% LA, 8%/h SDR; 3) high linoleic acid (HLA) = pH 6.5, 3% LA, 4%/h SDR; and 4) low pH (LPH) = pH 5.8, 1% LA, 4%/h SDR. Inoculum was collected 6 h after feeding from a cow fed 40% alfalfa hay and 60% grain. Liquid dilution rate was held at 0.12/h. All treatments except HLA contained 2% tallow. The LA was dissolved in buffer and continuously infused into the fermenters. The CLA flows were 16.5, 20.4, 23.2, and 25.2 mg/d for control, HSDR, HLA, and LPH, respectively. Compared with control, LPH increased flows of CLA, cis-C18:1, and C18:2, and decreased flow of C18:0. The neutral detergent fiber (NDF) and acid detergent fiber (ADF) digestibilities were not affected by pH. The HSDR tended to increase CLA flow compared to control, possibly because a shorter solid retention time led to incomplete biohydrogenatio...Continue Reading

References

Jul 1, 1994·Atherosclerosis·K N LeeM W Pariza
Feb 15, 1994·Biochemical and Biophysical Research Communications·C C MillerM E Cook
Jun 1, 1993·Journal of Dairy Science·D L PalmquistD M Barbano
Sep 1, 1995·Journal of Dairy Science·P J GaynorB B Teter
Mar 10, 1997·Biochimica Et Biophysica Acta·J L SébédioW W Christie
Jun 1, 1997·The Journal of Nutrition·P W Parodi
Aug 16, 1997·Biochimica Et Biophysica Acta·M C BarberR G Vernon
Jun 11, 1998·Journal of Dairy Science·J M GriinariK V Nurmela
Oct 26, 1999·Journal of Dairy Science·T R DhimanM W Pariza
Feb 23, 2000·European Psychiatry : the Journal of the Association of European Psychiatrists·S Cervera-EnguixS P McKenna
Apr 5, 2001·Journal of Dairy Science·P Y ChouinardD E Bauman
May 29, 2002·The Journal of Nutritional Biochemistry·Benjamin A. CorlDale E. Bauman

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Citations

Aug 1, 2010·Animal : an International Journal of Animal Bioscience·R MohammedJ J Murphy
Sep 25, 2015·Animal : an International Journal of Animal Bioscience·D E RicoR Gervais
Sep 23, 2014·The Veterinary Clinics of North America. Food Animal Practice·Thomas C Jenkins, Kevin J Harvatine
Mar 11, 2006·Journal of Dairy Science·J L FirkinsN R St-Pierre
May 1, 2008·Animal : an International Journal of Animal Bioscience·F GlasserM Doreau
Aug 21, 2008·Journal of Industrial Microbiology & Biotechnology·Anil K PuniyaKishan Singh
Feb 14, 2007·Journal of Dairy Science·C V D M RibeiroD L Palmquist

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