Effects of sulfanilamide and methotrexate on 13C fluxes through the glycine decarboxylase/serine hydroxymethyltransferase enzyme system in arabidopsis

Plant Physiology
V PrabhuJ King

Abstract

In C3 plants large amounts of photorespiratory glycine (Gly) are converted to serine by the tetrahydrofolate (THF)-dependent activities of the Gly decarboxylase complex (GDC) and serine hydroxymethyltransferase (SHMT). Using 13C nuclear magnetic resonance, we monitored the flux of carbon through the GDC/SHMT enzyme system in Arabidopsis thaliana (L.) Heynh. Columbia exposed to inhibitors of THF-synthesizing enzymes. Plants exposed for 96 h to sulfanilamide, a dihydropteroate synthase inhibitor, showed little reduction in flux through GDC/SHMT. Two other sulfonamide analogs were tested with similar results, although all three analogs competitively inhibited the partially purified enzyme. However, methotrexate or aminopterin, which are confirmed inhibitors of Arabidopsis dihydrofolate reductase, decreased the flux through the GDC/SHMT system by 60% after 48 h and by 100% in 96 h. The uptake of [alpha-13C]Gly was not inhibited by either drug class. The specificity of methotrexate action was shown by the ability of 5-formyl-THF to restore flux through the GDC/SHMT pathway in methotrexate-inhibited plants. The experiments with sulfonamides strongly suggest that the mitochondrial THF pool has a long half-life. The studies with methot...Continue Reading

References

Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D R Appling
May 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B I SchweitzerJ R Bertino
May 1, 1993·The Plant Journal : for Cell and Molecular Biology·G LazarH M Goodman
Mar 1, 1993·Trends in Biochemical Sciences·P Stover, V Schirch
Apr 19, 1996·The Journal of Biological Chemistry·M NeuburgerR Douce
Nov 1, 1996·Biochemical and Biophysical Research Communications·J HuangpuJ S Graham

❮ Previous
Next ❯

Citations

Sep 7, 2005·Photosynthesis Research·F Mesnard, R G Ratcliffe
Apr 29, 2000·Trends in Plant Science·A D HansonY Shachar-Hill
Jun 7, 2002·Trends in Plant Science·John King
Jan 19, 2000·Trends in Plant Science·J K Roberts
Apr 20, 2001·Current Opinion in Plant Biology·R Bligny, R Douce
Apr 19, 2002·Current Opinion in Plant Biology·Andrew D Hanson, Jesse F Gregory
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·J M MouillonF Rébeillé
May 22, 2013·Plant Physiology·Michael E StokesMalcolm M Campbell
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Andrew D Hanson, Sanja Roje
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·R George Ratcliffe, Yair Shachar-Hill
May 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Meena R ChandokDaniel F Klessig
Feb 1, 2011·Annual Review of Plant Biology·Andrew D Hanson, Jesse F Gregory
May 27, 2014·Applied Biochemistry and Biotechnology·Xiaomei ZhangZhenghong Xu
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Giuseppe OrsomandoAndrew D Hanson
Sep 19, 2002·The Journal of Biological Chemistry·Sanja RojeAndrew D Hanson
May 13, 2005·The Journal of Biological Chemistry·Aymeric GoyerAndrew D Hanson
Dec 24, 2002·The Journal of Biological Chemistry·Hieronim Jakubowski, Andrzej Guranowski
Apr 21, 2017·Frontiers in Chemistry·Vera GorelovaDominique Van Der Straeten
Apr 2, 2003·Plant & Cell Physiology·Rong LiJohn King
Jan 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·H A SultanNabeel A Raheem
Apr 2, 2021·The Plant Cell·Michael BuscheJacob O Brunkard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.