Effects of sulfur amino acids, L: -methionine, L: -cystine and L: -cysteine on lipoprotein lipase and hormone-sensitive lipase in differentiated mouse 3T3-L1 adipocytes.

Cytotechnology
M KawasakiK Yagasaki

Abstract

Rats subcutaneously implanted with AH109A hepatoma cells show hyperlipidemia with high concentrations of serum triglyceride and nonesterified fatty acid, suppression of lipoprotein lipase (LPL), and elevation of hormone-sensitive lipase (HSL) activities during the growth of the hepatoma. Supplementation of the diet with sulfur amino acids such as L: -methionine (Met) and L: -cystine (Cys) improved hyperlipidemia by restoring LPL and HSL activities. In the present study, we have attempted to examine the effects of sulfur amino acids on the activity and mRNA level of LPL and the activity of HSL using 3T3-L1 cells, which are known to differentiate to adipocytes. The adipocytes were incubated with various concentrations of Met, Cys or L: -cysteine (CysH) in the absence or presence of tumor necrosis factor-alpha (TNF-alpha). LPL activity was suppressed by TNF-alpha. In the absence of TNF-alpha, Met, Cys and CysH did not change the LPL activity. In the presence of TNF-alpha, Met and Cys significantly increased the LPL activity, and Met also enhanced the LPL mRNA level. HSL activity was also suppressed by TNF-alpha. In the absence of TNF-alpha, Met enhanced the HSL activity. In the presence of TNF-alpha, Met, Cys and CysH suppressed t...Continue Reading

References

Jan 1, 1979·Metabolism: Clinical and Experimental·T NakaiR Takeda
Dec 1, 1986·Journal of Nutritional Science and Vitaminology·K YagasakiR Funabiki
Mar 27, 1987·Science·K L WionR M Lawn
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M KawamuraD Steinberg
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M KawakamiA Cerami
Jan 28, 2004·Bioscience, Biotechnology, and Biochemistry·Masashi KawasakiRyuhei Funabiki
Aug 23, 2008·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·S MuntoniS Muntoni
Jan 9, 2010·Bioscience, Biotechnology, and Biochemistry·Masashi KawasakiKazumi Yagasaki

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Citations

May 21, 2014·Experimental Neurology·Kasper C D Roet, Joost Verhaagen
May 9, 2016·Amino Acids·Xihong ZhouYulong Yin
Feb 24, 2020·Ecotoxicology and Environmental Safety·Kai-Zhong XuZheng-Bing Guan

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