Effects of Sunphenon and Polyphenon 60 on proteolytic pathways, inflammatory cytokines and myogenic markers in H2O2-treated C2C12 cells

Journal of Biosciences
Allur Subramaniyan Sivakumar, Inho Hwang

Abstract

The effect of Sunphenon and Polyphenon 60 in oxidative stress response, myogenic regulatory factors, inflammatory cytokines, apoptotic and proteolytic pathways on H2O2-induced myotube atrophy was addressed. Cellular responses of H2O2-induced C2C12 cells were examined, including mRNA expression of myogenic regulatory factors, such as MyoD and myogenin, inflammatory pathways, such as TNF-α and NF-kB, as well as proteolytic enzymes, such as μ-calpain and m-calpain. The pre-treatment of Sunphenon (50 μg/mL)/Polyphenon 60 (50 μg/mL) on H2O2-treated C2C12 cells significantly down-regulated the mRNA expression of myogenin and MyoD when compared to those treated with H2O2-induced alone. Additionally, the mRNA expression of μ-calpain and m-calpain were significantly(p<0.05) increased in H2O2-treated C2C12 cells, whereas pre-treatment with Sunphenon/Polyphenon significantly down-regulated the above genes, namely μ-calpain and m-calpain. Furthermore, the mRNA expression of TNF-α and NF-kB were significantly increased in H2O2-treated C2C12 cells, while pre-treatment with Sunphenon (50 μg/mL)/Polyphenon 60 (50 μg/mL) significantly (p<0.05) down-regulated it when compared to the untreated control group.Subsequent analysis of DNA degeneration...Continue Reading

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Citations

May 20, 2016·Applied Biochemistry and Biotechnology·Sivakumar Allur SubramaniyanInho Hwang
Aug 29, 2017·Journal of Cachexia, Sarcopenia and Muscle·Jacob L BrownNicholas P Greene
Jun 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Noriyuki MiyoshiYukihiko Hara
Sep 13, 2020·Journal of Cachexia, Sarcopenia and Muscle·Jacob L BrownHolly Van Remmen
Jul 11, 2020·Biochimica Et Biophysica Acta. General Subjects·Prachi GuptaAshwani Mittal

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