PMID: 15347967Sep 7, 2004Paper

Effects of surface-treated cpTi and Ti6Al4V alloy on the initial attachment of human osteoblast cells

Journal of Materials Science. Materials in Medicine
M O OjiS Downes

Abstract

This study concerns the effect of simple surface treatments on the nature of the oxide layer, of commercially pure titanium (cpTi) and Ti6Al4V alloy substrates and their effect on human osteoblast cells (HOBS). After treatment the surfaces were analyzed by X-ray photoelectron spectroscopy (XPS) in order to identify the surface groups responsible for the cell attachment process. The assessment of cell attachment was monitored by the Alamar blue assay (AB), measuring cell activity, in three types of media: phosphate-buffered saline (PBS), serum containing and serum-free Dulbecco's modified Eagle's cell culture medium (SER+ and SERF respectively). XPS analysis of the treated surfaces revealed consistent peaks representative of TiO2 on all surfaces and Ti(0) and Ti2O3 on the non-heat-treated surfaces. The cell activity assays indicated that there were no significant differences in cellular activity caused by surface treatments, but the cellular activity compared between the three types of medium was greatest in the PBS over the initial stages of attachment.

Citations

May 12, 2006·Journal of Materials Science. Materials in Medicine·K Anselme, M Bigerelle
Feb 11, 2005·Journal of Biomedical Materials Research. Part a·L SaldañaL Munuera
Jun 30, 2007·Journal of Biomedical Materials Research. Part a·Naoki TsukimuraTakahiro Ogawa
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·Ryan J Love, Kim S Jones
Oct 20, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·S R SousaM A Barbosa

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