Abstract
Chlamydia trachomatis is a human pathogen that causes multiple diseases worldwide. Despite appropriate therapy with existing antichlamydial antibiotics, chronic exacerbated diseases often occur and lead to serious sequelae. Since C. trachomatis has been found to enter a persistent state after exposure to deleterious conditions, the role of persistence in the failure of chlamydial antibiotherapy is questioned. HeLa, THP-1 and U-937 cells were infected with 10(4)C. trachomatis serovar L2 infectious particles. Three days later the infected cells were treated with minimal bactericidal concentrations of doxycycline (DOX), erythromycin (ERY) or tetracycline (TET) for 24 days or 30 days. Antibiotic efficacy was assessed by measuring chlamydial inclusions and infectious particles, by investigating the resumption of chlamydial growth after antibiotic removal and by testing Chlamydia viability using reverse transcriptase polymerase chain reaction targeting unprocessed 16S rRNA, processed 16S rRNA and Omp-1 mRNA. Treatment of infected HeLa cells with the usual antichlamydial antibiotics suppressed chlamydial active growth. The infection remained unapparent. However, 24 days post treatment the bacterium was found to be viable, as proved by...Continue Reading
Citations
Jul 4, 2013·World Journal of Microbiology & Biotechnology·V I Hairul IslamS Ignacimuthu
Aug 21, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Antonietta RizzoMaria Rosaria Iovene
Jun 6, 2008·International Journal of Antimicrobial Agents·A R BhengrajA Mittal
Feb 28, 2006·Scandinavian Journal of Immunology·P MpigaM Ravaoarinoro
Mar 3, 2010·American Journal of Reproductive Immunology : AJRI·Alison J Carey, Kenneth W Beagley
Jun 19, 2013·Cell Host & Microbe·Cindrilla ChumduriThomas F Meyer
Nov 27, 2015·International Journal of STD & AIDS·E LanjouwM Unemo
Jan 11, 2017·Nature Reviews. Urology·Magnus Unemo, Jorgen S Jensen
Jul 31, 2009·Vascular and Endovascular Surgery·Annette HøghJes S Lindholt
Nov 27, 2015·International Journal of STD & AIDS·E LanjouwM Unemo
Jul 11, 2018·Expert Review of Molecular Diagnostics·Kevin J H JanssenPetra F G Wolffs
Apr 1, 2014·Journal of Bacteriology·Wilhelmina M HustonPeter Timms
Dec 7, 2018·BMJ Open·Jessica KrahnAmeeta E Singh
Jul 25, 2019·Frontiers in Microbiology·Hongliang ChenZhongyu Li