Effects of systematic peripheral group deuteration on the low-frequency resonance Raman spectra of myoglobin derivatives

Biopolymers
Piotr J MakL M Proniewicz

Abstract

Resonance Raman spectra are reported for a series of systematically deuterated analogues of myoglobin in its deoxy state as well as for its CO and O(2) adducts. Specifically, the myoglobin samples studied are those that have been reconstituted with deuterated protoheme analogues. These include the methine deuterated, protoheme-d4; analogue bearing C(2)H(3) groups at the 1, 3, 5, and 8 positions, protoheme-d12; the species bearing C(2)H(3) groups at the 1 and 3 positions only, 1,3-protoheme-d6; and the species bearing C(2)H(3) groups at the 5 and 8 positions only, 5,8-protoheme-d6. While the results are generally consistent with previously reported data for synthetic metalloporphyrin models and previous studies of labeled heme proteins, the high-quality low-frequency RR data reported here reveal several important aspects of these low-frequency modes. Of special interest is the fact that, using the two d6-protoheme analogues, it is shown that certain modes are apparently localized on particular pyrrole rings, while others are localized on different rings; i.e., several of these low-frequency modes are localized on one side of the heme.

References

Apr 1, 1975·Analytical Biochemistry·R K DiNello, D H Dolphin
Jan 1, 1992·Annual Review of Physical Chemistry·A D Procyk, D F Bocian
May 31, 1985·Biochemical and Biophysical Research Communications·M Barbush, D W Dixon
Jan 1, 1981·Methods in Enzymology·J B Wittenberg, B A Wittenberg
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·T K DasD L Rousseau
Dec 1, 1958·Biochimica Et Biophysica Acta·A R FANELLIA CAPUTO

❮ Previous
Next ❯

Citations

Jun 18, 2015·Biochemistry·Arianna I CelisJennifer L DuBois
Jun 3, 2018·The Journal of Biological Chemistry·Gareth K JenningsJohn C Hackett
Apr 30, 2016·Molecular Pharmacology·Gareth K JenningsJohn C Hackett
Apr 24, 2018·Journal of Inorganic Biochemistry·Francisco Zarate-PerezJohn C Hackett
Jul 3, 2017·Biochimica Et Biophysica Acta. Proteins and Proteomics·Piotr J Mak, Ilia G Denisov
Jun 13, 2008·The Journal of Physical Chemistry. a·Azaria S Eisenberg, Johannes P M Schelvis
Oct 3, 2018·Biochemistry·Bezalel A BaconElizabeth M Boon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.