Effects of temperature, agitation, and donor strain on chromosome transfer in Escherichia coli K-12.

Journal of Bacteriology
T H Wood

Abstract

Recombinant production in Escherichia coli K-12 can be described by three parameters: (i) the distance x of a selected male marker from the donor origin; (ii) the gradient constant k (the probability of interruption of the donor chromosome per unit distance during transfer into a recipient cell); and (iii) the extrapolate number A (the probability that a donor cell will produce a recombinant inheriting the donor marker contiguous with the origin). It is usually assumed that chromosomal distances can be measured by marker entry times, i.e., that the velocity of chromosome transfer v is constant along the chromosome. The dependencies of k, A, x, and v on temperature, agitation during mating, and donor strain were studied. The transfer velocity of the HfrH chromosomal region from the origin to his increases 15-fold between 16 and 43 C, and the chromosomal regions studied have the same temperature dependence that was found for the separate transfer velocities of the O-trp and trp-his regions. These data and radiation studies on chromosome transfer indicate that, at a given temperature, chromosomal transfer velocity varies by less than 10% as the distance of any given region from the origin increases. The gradient constant k is temp...Continue Reading

References

Dec 1, 1967·Bacteriological Reviews·A L Taylor, C D Trotter
Aug 1, 1967·Journal of Bacteriology·R Curtiss, D R Stallions
Feb 1, 1957·Journal of General Microbiology·W HAYES
Jan 1, 1958·Cold Spring Harbor Symposia on Quantitative Biology·T F ANDERSON
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Jul 1, 1965·Genetical Research·B LOW, T H WOOD

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Citations

Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
Feb 1, 1974·Journal of Bacteriology·P Broda, J F Collins
Jul 1, 1972·Journal of Bacteriology·J T Ou, T F Anderson
Jun 1, 1970·Journal of Bacteriology·N Bouck, E A Adelberg
Jan 1, 1976·Journal of Bacteriology·M S Guyer, A J Clark
Sep 1, 1970·Journal of Bacteriology·M WannT H Wood
Nov 1, 1969·Mutation Research·P G De HaanH S Felix
May 1, 1976·Journal of Bacteriology·L M Havekes, W P Hoekstra
Jun 15, 1973·Journal of Molecular Biology·O Siddiqi, M S Fox
Mar 14, 1970·Journal of Molecular Biology·E E Moody, A Lukin

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