PMID: 9635776Jun 23, 1998Paper

Effects of temperature and deltaGo on electron transfer from cytochrome c2 to the photosynthetic reaction center of the purple bacterium Rhodobacter sphaeroides

Biophysical Journal
G VenturoliP Mathis

Abstract

The kinetics of electron transfer from cytochrome c2 to the primary donor (P) of the reaction center from the photosynthetic purple bacterium Rhodobacter sphaeroides have been investigated by time-resolved absorption spectroscopy. Rereduction of P+ induced by a laser pulse has been measured at temperatures from 300 K to 220 K in a series of specifically mutated reaction centers characterized by altered midpoint redox potentials of P+/P varying from 410 mV to 765 mV (as compared to 505 mV for wild type). Rate constants for first-order electron donation within preformed reaction center-cytochrome c2 complexes and for the bimolecular oxidation of free cytochrome c2 have been obtained by multiexponential deconvolution of the kinetics. At all temperatures the rate of the fastest intracomplex electron transfer increases by more than two orders of magnitude as the driving force -deltaGo is varied over a range of 350 meV. The temperature and deltaGo dependences of the rate constant fit the Marcus equation well. Global analysis yields a reorganization energy lambda = 0.96 +/- 0.07 eV and a set of electronic matrix elements, specific for each mutant, ranging from 1.2 10(-4) eV to 2.5 10(-4) eV. Analysis in terms of the Jortner equation i...Continue Reading

References

Sep 11, 1979·Biochimica Et Biophysica Acta·R C Prince, C L Bashford
Feb 27, 1992·Nature·C C MoserP L Dutton
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M R Gunner, B Honig
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P AllenD C Rees
Jun 26, 1987·Science·A Kuki, P G Wolynes
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·X LinJ C Williams
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J J Hopfield

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Citations

Jun 7, 2006·Photosynthesis Research·Giorgio FortiGiuseppe Zucchelli
Jan 30, 2007·Biophysical Journal·Ilia A Solov'yovKlaus Schulten
Sep 18, 2012·The International Journal of Biochemistry & Cell Biology·Marina Verkhovskaya, Dmitry A Bloch
Oct 28, 2009·The Journal of Physical Chemistry. B·Mário Fragata, Venkataramanaiah Viruvuru
Sep 26, 2009·Biochemistry·Stefano SantabarbaraFabrice Rappaport
May 4, 2011·Journal of the American Chemical Society·Agnieszka A ZiebaJohannes P M Schelvis
Feb 3, 2007·The Journal of Physical Chemistry. B·Martin RegehlyBeate Röder

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