Effects of temperature and storage conditions on the electrophoretic, toxic and enzymatic stability of venom components

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Sean M Munekiyo, S P Mackessy

Abstract

Rattlesnake venoms are complex biological products containing potentially autolytic components, and they provide a useful tool for the study of long-term maintenance of enzymes in a competent state, both in vivo and in vitro. To evaluate the stability of venom components, 15 aliquots of freshly extracted venom (from Crotalus molossus molossus) were subjected to 15 different temperature and storage conditions for 1 week and then lyophilized; conditions varied from storage at -80 degrees C (optimal preservation of activities) to dilution (1:24) and storage at 37 degrees C (maximal degradation potential). Effects of different storage conditions were evaluated using SDS-PAGE, metalloprotease zymogram gels, a cricket LD50 assay and enzyme assays (metalloprotease, serine proteases, phosphodiesterase, L-amino acid oxidase and phospholipase A2). Venom samples were remarkably refractive to widely varying conditions; enzyme activities of some samples were variable, particularly L-amino acid oxidase, and one sample treatment showed higher toxicity, but electrophoretic results indicated very little effect on venom proteins. This study suggests that most venom activities should remain stable even if stored or collected under potentially adv...Continue Reading

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Citations

Jun 20, 2000·Toxicon : Official Journal of the International Society on Toxinology·R E Hill, S P Mackessy
Mar 5, 2013·BMC Biology·Anthony J SaviolaStephen P Mackessy
Jan 18, 2014·Journal of Proteomics·Clémence JesupretBryan Grieg Fry
Jul 2, 2010·Wilderness & Environmental Medicine·William K Hayes, Stephen P Mackessy
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Sean M Munekiyo, Stephen P Mackessy
Mar 21, 2006·Toxicon : Official Journal of the International Society on Toxinology·Stephen P MackessyThomas Fritts
Dec 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Nihal M IbrahimAhmed F Wahby
Jul 6, 2014·Chemico-biological Interactions·Jaime Andrés PereañezEdison Osorio
Apr 26, 2016·International Journal of Biological Macromolecules·Isabel Gómez-BetancurDora Benjumea
Sep 18, 2016·Integrative and Comparative Biology·Stephen P Mackessy, Anthony J Saviola
Mar 1, 2017·Journal of Biochemical and Molecular Toxicology·Wafa TachouaFatima Laraba-Djebari
Nov 23, 2007·Natural Product Reports·Yonghong LiuSi Zhang
Apr 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Octavio Roldán-PadrónTeresa García-Gasca
Dec 12, 2020·Toxicon : Official Journal of the International Society on Toxinology·William H Heyborne, Stephen P Mackessy
Jan 28, 2021·Toxicon : Official Journal of the International Society on Toxinology·Ricardo S P PatiñoJosé R Almeida
Sep 9, 2020·Toxicon : Official Journal of the International Society on Toxinology·Iván Fernando Pozas-OcampoGustavo Arnaud-Franco
Apr 8, 2019·Toxicon : Official Journal of the International Society on Toxinology·Christopher J Rex, Stephen P Mackessy
Mar 17, 2010·Toxicon : Official Journal of the International Society on Toxinology·Stephen P Mackessy

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