Effects of temperature on survival, moulting, and expression of neuropeptide and mTOR signalling genes in juvenile Dungeness crab (Metacarcinus magister )

The Journal of Experimental Biology
Astrid C WittmannDonald L Mykles

Abstract

Mechanistic target of rapamymcin (mTOR) is a highly conserved protein kinase that controls cellular protein synthesis and energy homeostasis. We hypothesize that mTOR integrates intrinsic signals (moulting hormones) and extrinsic signals (thermal stress) to regulate moulting and growth in decapod crustaceans. The effects of temperature on survival, moulting and mRNA levels of mTOR signalling genes (Mm-Rheb, Mm-mTOR, Mm-AMPKα, Mm-S6K and Mm-AKT) and neuropeptides (Mm-CHH and Mm-MIH) were quantified in juvenile Metacarcinus magister Crabs at different moult stages (12, 19 or 26 days postmoult) were transferred from ambient temperature (∼15°C) to temperatures between 5 and 30°C for up to 14 days. Survival was 97-100% from 5 to 20°C, but none survived at 25 or 30°C. Moult stage progression accelerated from 5 to 15°C, but did not accelerate further at 20°C. In eyestalk ganglia, Mm-Rheb, Mm-AMPKα and Mm-AKT mRNA levels decreased with increasing temperatures. Mm-MIH and Mm-CHH mRNA levels were lowest in the eyestalk ganglia of mid-premoult animals at 20°C. In the Y-organ, Mm-Rheb mRNA levels decreased with increasing temperature and increased during premoult, and were positively correlated with haemolymph ecdysteroid titre. In the hea...Continue Reading

References

Apr 21, 1998·Molecular and Cellular Endocrinology·H R UmphreyE Spaziani
Sep 10, 1998·Biochemical and Biophysical Research Communications·K J LeeR D Roer
Aug 9, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·D StorchH-O Pörtner
Apr 28, 2005·Current Biology : CB·Xiaomeng LongJoseph Avruch
Jun 9, 2005·The Journal of Experimental Biology·Daniela StorchHans O Pörtner
Apr 29, 2006·The Journal of Physiology·D Grahame HardieJohn W Scott
Oct 17, 2006·Oncogene·J H Reiling, D M Sabatini
Oct 16, 2008·Developmental Cell·Sophie LayallePierre Léopold
Feb 17, 2009·The Journal of Experimental Biology·Markus FrederichJennifer A Jost
Sep 15, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Donald L Mykles
Dec 24, 2010·Nature·Shomit SenguptaDavid M Sabatini
Feb 4, 2011·Insect Biochemistry and Molecular Biology·R Parthasarathy, Subba R Palli
Apr 20, 2011·General and Comparative Endocrinology·Ernest S Chang, Donald L Mykles
Jul 5, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Michael MartinDirk Weihrauch
Aug 26, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Heidrun Sigrid WindischMagnus Lucassen
Sep 29, 2011·The Journal of Physiology·Craig A GoodmanTroy A Hornberger
Apr 13, 2012·Physiological and Biochemical Zoology : PBZ·Astrid C WittmannFranz J Sartoris
Feb 26, 2013·Physiological and Biochemical Zoology : PBZ·Katja AnttilaAnthony P Farrell
Oct 22, 2013·G3 : Genes - Genomes - Genetics·Stefanie JarolimIan W Dawes
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Patrick A GibneyDavid Botstein
Feb 1, 2014·Circulation Research·Sebastiano SciarrettaJunichi Sadoshima
Feb 22, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Zhaoying QianXiaolin Liu
Jun 7, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·H Frederik NijhoutViviane Callier
Jul 6, 2014·Science Signaling·Jose AramburuCristina López-Rodríguez
Dec 23, 2015·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Sunetra DasDonald L Mykles
Feb 3, 2016·Frontiers in Physiology·Adam N KeenHolly A Shiels

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Jul 9, 2021·Frontiers in Endocrinology·Donald L Mykles

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