Effects of tert-butyl hydroperoxide on promotable and non-promotable JB6 mouse epidermal cells

Chemico-biological Interactions
D MuehlematterP Cerutti

Abstract

Oxidants and agents that induce a cellular prooxidant state can act as carcinogens. We compared the effect of tert-butyl hydroperoxide (Bu-OOH) on DNA strand breakage, poly ADP-ribosylation of chromosomal proteins and the expression of the proto-oncogenes c-fos and c-myc between non-promotable clone 30 and promotable clone 41 of mouse epidermal cells JB6. These pathophysiological effects of oxidants are mechanistically related. Bu--OOH caused more DNA-strand breakage at high concentrations and more extensive poly ADP-ribose accumulation in clone 30 than in clone 41, in reactions which require intracellular free iron. Clone 41 exhibited constitutive c-myc expression while c-fos mRNA was very low in untreated cultures of both clones. Low concentrations of Bu-OOH induced c-myc and more strongly c-fos in clone 41. Both proto-oncogenes were strongly induced in clone 30. Our results allow insights into the mechanisms of action of a typical organic hydroperoxide in JB6 cells. However, they do not uncover the reasons for the differential promotability of the two JB6 clones by oxidants beyond the implication of the constitutive expression of c-myc in promotable clone 41.

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Citations

Jan 1, 1991·Free Radical Biology & Medicine·M A Baker, S Q He
Nov 21, 1992·Annals of the New York Academy of Sciences·P CeruttiP Amstad
Apr 6, 1999·Carcinogenesis·R P HuangA L Boynton
Apr 22, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·William A PryorKelvin J A Davies
Jan 5, 2002·Physiological Reviews·Wulf Dröge
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Nov 11, 1991·International Journal of Cancer. Journal International Du Cancer·D R Crawford, J S Greenberger
Jul 19, 2021·Free Radical Biology & Medicine·Joyce BagyiDana R Crawford

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