Effects of testosterone treatment on markers of skeletal muscle ribosome biogenesis

Andrologia
C Brooks MobleyMichael D Roberts

Abstract

The effects of testosterone (TEST) treatment on markers of skeletal muscle ribosome biogenesis in vitro and in vivo were examined. C2 C12 myotubes were treated with 100 nm TEST for short-term (24-h) and longer-term (96-h) treatments. Moreover, male 10-month-old Fischer 344 rats were housed for 4 weeks, and the following groups were included in this study: (i) Sham-operated (Sham) rats, (ii) orchiectomised rats (ORX) and (iii) ORX+TEST-treated rats (7.0 mg week(-1) ). For in vitro data, TEST treatment increased c-Myc mRNA expression by 38% (P = 0.004) after 96 h, but did not affect total RNA, 47S pre-rRNA, Raptor mRNA, Nop56 mRNA, Bop1 mRNA, Ncl mRNA at 24 h or 96 h following the treatment. For in vivo data, ORX decreased levator ani/bulbocavernosus (LABC) myofibril protein versus Sham (P = 0.006), whereas ORX+TEST (P = 0.015) rescued this atrophic effect. ORX also decreased muscle ribosome content (total RNA) compared to Sham (P = 0.046), whereas ORX+TEST tended to rescue this effect (P = 0.057). However, other markers of ribosome biogenesis including c-Myc mRNA, Nop56 mRNA, Bop1 mRNA, Ncl mRNA decreased with ORX independently of TEST treatments (P < 0.05). Finally, lower phospho-(Ser235/236)-to-total rps6 protein and lower rpl...Continue Reading

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Citations

Jun 2, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Michael L Rossetti, Bradley S Gordon
Jun 14, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael L RossettiBradley S Gordon
Aug 20, 2016·Journal of Applied Physiology·A Maleah HollandDarren T Beck
Sep 14, 2017·American Journal of Physiology. Endocrinology and Metabolism·Bradley S GordonPaul M Coen
Jul 31, 2019·American Journal of Physiology. Endocrinology and Metabolism·Michael L RossettiBradley S Gordon
Mar 29, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Francisco CastelánMargarita Martínez-Gómez
Dec 10, 2020·Scientific Reports·Yehan FangJianping Lin
Mar 7, 2021·Mayo Clinic Proceedings·Mark W PatakyK Sreekumaran Nair
Oct 31, 2020·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Bradley S GordonRobert A Casero

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