Effects of tetracaine on voltage-activated calcium sparks in frog intact skeletal muscle fibers

The Journal of General Physiology
S HollingworthS M Baylor

Abstract

The properties of Ca(2+) sparks in frog intact skeletal muscle fibers depolarized with 13 mM [K(+)] Ringer's are well described by a computational model with a Ca(2+) source flux of amplitude 2.5 pA (units of current) and duration 4.6 ms (18 degrees C; Model 2 of Baylor et al., 2002). This result, in combination with the values of single-channel Ca(2+) current reported for ryanodine receptors (RyRs) in bilayers under physiological ion conditions, 0.5 pA (Kettlun et al., 2003) to 2 pA (Tinker et al., 1993), suggests that 1-5 RyR Ca(2+) release channels open during a voltage-activated Ca(2+) spark in an intact fiber. To distinguish between one and greater than one channel per spark, sparks were measured in 8 mM [K(+)] Ringer's in the absence and presence of tetracaine, an inhibitor of RyR channel openings in bilayers. The most prominent effect of 75-100 microM tetracaine was an approximately sixfold reduction in spark frequency. The remaining sparks showed significant reductions in the mean values of peak amplitude, decay time constant, full duration at half maximum (FDHM), full width at half maximum (FWHM), and mass, but not in the mean value of rise time. Spark properties in tetracaine were simulated with an updated spark model...Continue Reading

References

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Citations

Aug 29, 2007·The Journal of General Physiology·Stephen M Baylor, Stephen Hollingworth
Mar 28, 2012·The Journal of General Physiology·Stephen M Baylor, Stephen Hollingworth
Jan 10, 2014·American Journal of Respiratory Cell and Molecular Biology·Oliver FriedrichMirko Völkers
Oct 26, 2010·Journal of Molecular and Cellular Cardiology·Sabine HukeDonald M Bers
Feb 11, 2015·The Journal of General Physiology·Stefano PerniClara Franzini-Armstrong
Dec 21, 2017·The Journal of General Physiology·Frank E NelsonLawrence C Rome
Oct 17, 2008·Physiological Reviews·Heping Cheng, W J Lederer
Oct 1, 2009·Physiological Reviews·Makoto Endo
Oct 14, 2011·Journal of Muscle Research and Cell Motility·Christopher L-H HuangJames A Fraser
Sep 29, 2011·The Journal of Physiology·Claire L HarwoodLawrence C Rome
Nov 30, 2011·The Journal of Physiology·Stephen HollingworthStephen M Baylor
Jul 6, 2010·Progress in Biophysics and Molecular Biology·Stephen M Baylor, Stephen Hollingworth

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