PMID: 11339506May 8, 2001Paper

Effects of tetracyclines on the production of matrix metalloproteinases and plasminogen activators as well as of their natural inhibitors, tissue inhibitor of metalloproteinases-1 and plasminogen activator inhibitor-1

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
T Sadowski, Jürgen Steinmeyer

Abstract

Evaluation of tetracycline effects on the expression of MMP-1, MMP-3, tissue inhibitor(s) of metalloproteinase-1 (TIMP-1), plasminogen activators (PA), and PA inhibitor-1, which are all involved in the ultimate regulation of MMP activity could provide new insight into how tetracyclines achieve their cartilage preserving effects. We used bovine articular chondrocytes cultured in alginate gel beads for our studies which were initially treated with 10 microM tetracyclines in the presence of IL-1. Only significant effects were studied at additional concentrations. Expression of mRNA was analyzed by RT-PCR-ELISA. The activity of enzymes and TIMP was measured by functional assays; whereas, the level of PAI-1 was determined by ELISA. Treating chondrocytes with IL-1 induced the expression of MMPs and downregulated TIMP-1 but stimulated both the expression of PAs and PAI-1. When tested at 10 microM only minocycline reduced collagenase activity and expression of MMP-1. Further pharmacokinetic analysis revealed IC50 values of 26 microM and 16 microM for the inhibition of collagenase activity and mRNA expression, respectively. Production of MMP-3 was only decreased by tetracycline (IC50 = 45.4 microM). No effects of tetracyclines could be ...Continue Reading

Citations

Oct 7, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Nurit MathaloneOrna Geyer
Oct 20, 2011·The Journal of Antimicrobial Chemotherapy·Riana CockeranRonald Anderson
Mar 5, 2009·Antimicrobial Agents and Chemotherapy·Chika IshikawaKiyofumi Yamanishi
Jan 15, 2014·International Journal of Pediatric Otorhinolaryngology·Chul Ho JangMin Young Kim
Oct 23, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·N Garrido-MesaJ Gálvez
Jan 8, 2004·The Journal of Investigative Dermatology·Thorsten SadowskiRadislav Sedlacek
Dec 15, 2006·Neurobiology of Disease·Dorothe BurggrafGerhard F Hamann
Apr 22, 2005·Pharmacotherapy·Susan C FaganAdviye Ergul
Oct 9, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lisa A FortierKaren G Mayr
May 22, 2010·Equine Veterinary Journal·L V SchnabelL A Fortier
Oct 8, 2004·Expert Opinion on Therapeutic Targets·Andrew D Cook, Kumar Visvanathan
Jun 27, 2002·Expert Opinion on Pharmacotherapy·Vadivelu Saravanan, Jennifer Hamilton
Nov 29, 2014·Archives of Medical Research·Genaro Gabriel OrtizMoisés Alejandro Alatorre-Jiménez
Nov 4, 2004·Current Opinion in Ophthalmology·Donald U Stone, James Chodosh
Feb 13, 2019·Brain Injury : [BI]·Jay MeythalerP Tyler Roskos
Mar 1, 2006·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Cord SunderkötterUta Jappe
Sep 30, 2017·International Journal of Oral Science·Kátia do Nascimento GomesGlauce Socorro de Barros Viana
Dec 11, 2003·Multiple Sclerosis : Clinical and Laboratory Research·Alireza Minagar, J Steven Alexander
Jul 12, 2020·International Journal of Environmental Research and Public Health·Vittorio ChecchiAnnalisa Mazzoni
Apr 13, 2021·Biochemical Society Transactions·David J Wilkinson
Sep 10, 2008·Journal of Agricultural and Food Chemistry·M Gratacós-CubarsíF Jessen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Phytotherapy Research : PTR
Gabriela MojžišováJán Richnavský
Klinische Monatsblätter für Augenheilkunde
T SchromG Duncker
American Journal of Otolaryngology
Manish GuptaTarun Narang
© 2021 Meta ULC. All rights reserved