Abstract
While previous studies have indicated that exogenous TGF-alpha stimulates epithelial growth, maintenance, and repair of the gut, roles of endogenous TGF-alpha are less well-defined particularly in the small bowel. The current study examined effects of TGF-alpha knockout on adult small intestinal epithelial cell proliferation, migration, apoptosis, and damage/repair response after methotrexate treatment. Compared to normal mice, TGF-alpha gene knockout did not affect crypt cell production, mitosis position, migration, and apoptosis in non-injured intestine. RT-PCR gene expression analysis revealed presence of four out of six TGF-alpha related EGF family ligands in the normal intestine, suggesting a possible functional redundancy of the EGF family in maintenance of the intestine. Although TGF-alpha gene knockout did not significantly impair the overall mucosal repair in methotrexate-induced acute damage in the small intestine, it resulted in a higher apoptotic response in the early hours following methotrexate challenge, and a delayed and reduced crypt cell proliferation during repair. Consistently, after methotrexate challenge, intestinal TGF-alpha mRNA was found to be markedly upregulated in the early hours and during repair in...Continue Reading
References
Dec 1, 1992·The Journal of Clinical Investigation·M RomanoR J Coffey
Jan 1, 1992·Progress in Growth Factor Research·S A Prigent, N R Lemoine
Aug 1, 1992·Scandinavian Journal of Gastroenterology·S J KonturekB L Slomiany
May 11, 1992·Gastroenterology·W H PolkR J Coffey
May 1, 1992·Gut·D M ThomasM R Alison
May 1, 1989·The Journal of Clinical Investigation·S Y Koyama, D K Podolsky
Mar 15, 1989·International Journal of Cancer. Journal International Du Cancer·L T MaldenA W Burgess
Nov 1, 1980·Cell and Tissue Kinetics·N A Wright, D R Appleton
Jun 1, 1980·Gut·J A TaminiauJ R Hamilton
Nov 10, 1994·Nature·E ArakiC R Kahn
Jun 1, 1995·Gut·C S PottenL B Woolford
Jul 27, 1995·Nature·P J MiettinenR Derynck
Apr 1, 1995·The Journal of Pathology·M R AlisonN A Wright
Feb 1, 1995·Gastroenterology·J A BarnardR J Coffey
Dec 1, 1994·Cell and Tissue Research·K Hormi, T Lehy
Jul 1, 1994·Gastroenterology·F ProcaccinoV E Eysselein
Apr 23, 1993·Cell·G B MannA R Dunn
Apr 23, 1993·Cell·N C LuettekeD C Lee
May 1, 1993·Pediatric Research·P J Miettinen
Feb 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S UmemuraY Tsutsumi
May 1, 1996·Gut·A U DignassM W Babyatsky
Oct 11, 1996·Science·H MashimoM C Fishman
Sep 1, 1997·Trends in Genetics : TIG·J CookeJ Maynard-Smith
Sep 1, 1997·Gastroenterology·B EggerV E Eysselein
Aug 1, 1997·Gut·P HoffmannV E Eysselein
Apr 17, 1998·European Journal of Pharmacology·P C KonturekE G Hahn
Aug 26, 1998·Growth Factors·G S HowarthL C Read
Dec 30, 1998·International Journal of Radiation Oncology, Biology, Physics·A C RuifrokH D Thames
Jun 22, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J XianL C Read
Feb 29, 2000·Journal of Pediatric Surgery·R A FalconeB W Warner
Mar 25, 2000·British Journal of Cancer·C J XianN C Kallincos
Jul 5, 2001·Gastroenterology·K L TroyerD C Lee
Citations
Mar 28, 2006·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Rajesh V LallaDorothy Keefe
Feb 11, 2005·Current Opinion in Gastroenterology·Julian R F Walters
Jan 20, 2009·World Journal of Gastroenterology : WJG·Laurie Drozdowski, Alan B R Thomson
Sep 18, 2010·Journal of Gastroenterology·Hiroki TanakaYasuhisa Shinomura
Mar 5, 2016·Journal of Ethnopharmacology·Cheng-Jin ShiCory J Xian
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mi-Hyun KangYoungsook Son
Sep 22, 2007·Bone·Cory J XianBruce K Foster
Aug 5, 2006·Journal of Cellular Biochemistry·Cory J XianBruce K Foster
Sep 6, 2007·Journal of Cellular Physiology·Cory J XianBruce K Foster
Jul 31, 2008·Journal of Pediatric Gastroenterology and Nutrition·Igor SukhotnikRaanan Shamir
Jan 22, 2010·Growth Factors·Sylvia H TrangR Lor Randall
Feb 27, 2007·Endocrine Reviews·Cory J Xian
Aug 15, 2013·British Journal of Pharmacology·Tatsuro NakamuraHiroshi Ozaki
Jan 24, 2019·Mediators of Inflammation·Sandra Rayego-MateosMarta Ruiz-Ortega
Jan 13, 2021·Biomedicines·Anna BilleschouHannelouise Kissow
Sep 1, 2004·Bone·C J XianB K Foster
Jun 18, 2015·Parasites & Vectors·Alba CortésRafael Toledo