PMID: 30014638Jul 18, 2018Paper

Effects of Thapsigargin on Intracellular Ca2+ and Caspase-3 Protein Expression in Mouse Lung Fibroblast

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Yu YanYong-hong Xiao

Abstract

To explore the effects of calcium pump inhibitor thapsigargin (TG) on intracellular Ca2+ and the expression of caspase-3 protein in mouse lung fibroblast. Mice lung fibroblast cells were divided into three groups: the blank control group,transforming growth factor-β1 (TGF-β1) group and TG treated group. The cells were induced by 5 ng/mL TGF-β1 for 24 h in TGF-β1 group and TG group,4 μmol/L TG was added in TG group for 24 h. After 48 h,the cells were collected,and the cell structure was observed by transmission electron microscope,intracellular Ca2+ level was detected with laser confocal microscope,the protein expression of caspase-3 was examined using immunohistochemistry method. Transmission electron microscopy showed that the cells in the blank control group had obvious nucleolus,complete organelles and less apoptosis. In TGF-β1 group,the cell morphology was intact,chromatin was evenly distributed,and no apoptotic cells were found. In TG group,there were a large number of apoptosis of fibroblasts,chromatin clumps in nuclei and a small amount of collagen fibers. The level of Ca2+ in TGF-β1 group was significantly lower than that in control group (P<0.05),and which in TG group was significantly higher (P<0.05 ). The protein exp...Continue Reading

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