Effects of the 1, 4-dihydropyridine L-type calcium channel blocker benidipine on bone marrow stromal cells

Cell and Tissue Research
Zhong-ping MaDao-zhang Cai

Abstract

Osteoporosis (OP) often increases the risk of bone fracture and other complications and is a major clinical problem. Previous studies have found that high blood pressure is associated with bone formation abnormalities, resulting in increased calcium loss. We have investigated the effect of the antihypertensive drug benidipine on bone marrow stromal cell (BMSC) differentiation into osteoblasts and bone formation under osteoporotic conditions. We used a combination of in vitro and in vivo approaches to test the hypothesis that benidipine promotes murine BMSC differentiation into osteoblasts. Alkaline phosphatase (ALP), osteocalcin (OCN), runt-related transcription factor 2 (RUNX2), β-catenin, and low-density lipoprotein receptor-related protein 5 (LRP5) protein expression was evaluated in primary femoral BMSCs from C57/BL6 mice cultured under osteogenic conditions for 2 weeks to examine the effects of benidipine. An ovariectomized (OVX) mouse model was used to investigate the effect of benidipine treatment for 3 months in vivo. We found that ALP, OCN, and RUNX2 expression was up-regulated and WNT/β-catenin signaling was enhanced in vitro and in vivo. In OVX mice that were intragastrically administered benidipine, bone parameters ...Continue Reading

References

Sep 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L J MeltonB L Riggs
Jan 1, 1988·Journal of Cell Science. Supplement·M Owen
Dec 1, 1985·Calcified Tissue International·Y IzawaT Makita
Aug 1, 1983·Clinical Science·P StrazzulloM Mancini
Oct 28, 1999·Journal of Cellular Biochemistry·J P RodríguezG Seitz
Jun 20, 2001·Biological & Pharmaceutical Bulletin·Y Nishiya, S Sugimoto
Nov 24, 2001·Cell·Y GongUNKNOWN Osteoporosis-Pseudoglioma Syndrome Collaborative Group
Feb 28, 2002·Gerontology·George K Chan, Gustavo Duque
May 17, 2002·The New England Journal of Medicine·Lynn M BoydenRichard P Lifton
May 16, 2003·Nature·Gerard Karsenty
May 16, 2003·Nature·Henry M Kronenberg
Dec 4, 2004·Development·Hongliang HuFanxin Long
Dec 5, 2006·Methods in Enzymology·Paolo BiancoPamela Gehron Robey
Sep 1, 2007·The New England Journal of Medicine·Ernesto CanalisJohn P Bilezikian
Jun 12, 2013·Calcified Tissue International·Maurizio RossiniSilvano Adami
Jun 12, 2013·Journal of Biochemistry·Lynett Danks, Hiroshi Takayanagi
Feb 13, 2014·Experimental and Therapeutic Medicine·Baixiang WangJingyun Wang

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Citations

May 23, 2019·Cell Proliferation·Yi-Zhou TanBei Li
Mar 12, 2021·Biochemical and Biophysical Research Communications·Bin ZhouYijun Kang
May 12, 2021·Journal of Zhejiang University. Science. B·Baixiang WangHuiming Wang

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