Effects of the bacterial transcription-repair coupling factor during transcription of DNA containing non-bulky lesions.

DNA Repair
Abigail J Smith, Nigel J Savery

Abstract

Transcription-coupled DNA repair is a mechanism by which bulky DNA lesions that block transcription by RNA polymerase are prioritised for removal by the nucleotide excision repair apparatus. The trigger is thought to be the presence of an irreversibly blocked transcription complex, which is recognised by a transcription-repair coupling factor. Many common DNA lesions do not block transcription, but are bypassed with varying degrees of efficiency and with potentially mutagenic effects on the RNA transcripts that are produced. The effect of the bacterial transcription-repair coupling factor, Mfd, at such lesions is not known: it has been suggested that Mfd may promote mutagenesis by increasing the efficiency with which RNA polymerase bypasses non-bulky lesions, but it has also been reported that 8-oxoguanine, a major product of oxidative DNA damage that is efficiently bypassed by RNA polymerase, is subject to Mfd-dependent transcription-coupled repair in Escherichia coli. We have investigated the effect of Mfd during transcription of templates containing 8-oxoguanine, and various other non-bulky lesions. We show that an 8-oxoguanine in the template strand induces a transient pause in transcription, and that Mfd neither increases ...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Kunala, D E Brash
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A SellittiD A Steege
Mar 3, 1995·The Journal of Biological Chemistry·C P Selby, A Sancar
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W Zhou, P W Doetsch
Apr 2, 1993·Science·C P Selby, A Sancar
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·N Komissarova, M Kashlev
Apr 24, 1999·Biochimie·S Tornaletti, P C Hanawalt
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·I Artsimovitch, R Landick
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karen AdelmanMichelle D Wang
Oct 29, 2003·Molecular Cell·Damien BrégeonPaul W Doetsch
Nov 7, 2003·Nucleic Acids Research·A L ChambersN J Savery
Oct 12, 2004·DNA Repair·David A ScicchitanoAlexandra Dimitri
Feb 3, 2005·Nucleic Acids Research·A J Smith, N J Savery
Apr 14, 2005·Journal of Molecular Biology·Stuart R W BellamyStephen E Halford
Nov 29, 2005·Journal of Molecular Biology·Nora AssenmacherKarl-Peter Hopfner
Feb 9, 2006·Chemical Reviews·James J TruglioCaroline Kisker
Feb 14, 2006·Cell·Alexandra M DeaconescuSeth A Darst
Mar 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joo-Seop Park, Jeffrey W Roberts
Jun 27, 2006·Trends in Genetics : TIG·Jean-Philippe Lainé, Jean-Marc Egly
Sep 5, 2006·Journal of Bacteriology·Christian RossEduardo Robleto
Sep 19, 2006·Chemical Research in Toxicology·Silvia TornalettiPhilip C Hanawalt
Nov 1, 2006·Biochemical Society Transactions·R Landick
Nov 18, 2006·The EMBO Journal·Nicolas Charlet-BerguerandJean Marc Egly
Jun 19, 2007·Trends in Microbiology·Nigel J Savery
Jan 1, 2003·Clinical Linguistics & Phonetics·Martin J Ball, Thomas W Powell

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Citations

Mar 5, 2013·Cold Spring Harbor Perspectives in Biology·Caroline KiskerBennett Van Houten
Jan 18, 2012·Journal of Molecular Microbiology and Biotechnology·Holly Anne MartinEduardo A Robleto
Jul 11, 2014·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Minako YoshiharaShinya Toyokuni
Oct 23, 2012·Trends in Biochemical Sciences·Alexandra M DeaconescuNikolaus Grigorieff
Jun 10, 2017·Transcription·Alexandra M Gehring, Thomas J Santangelo
May 23, 2020·The Journal of Biological Chemistry·Aleksei AgapovAndrey Kulbachinskiy
Jun 29, 2013·Cellular and Molecular Life Sciences : CMLS·Alexandra M Deaconescu
Jul 4, 2019·MBio·Veronica NegroZeynep Baharoglu
Jul 21, 2016·Scientific Reports·Elisabeth GuillemetNalini Ramarao
Aug 17, 2019·RNA Biology·Aleksei AgapovAndrey Kulbachinskiy
Aug 12, 2018·Journal of Molecular Biology·Tung T Le, Michelle D Wang
Feb 20, 2021·Frontiers in Microbiology·Holly Anne MartinEduardo A Robleto

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