PMID: 2501938Mar 1, 1989Paper

Effects of the fungicide prochloraz on xenobiotic metabolism in rainbow trout: inhibition in vitro and time course of induction in vivo

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
J Snegaroff, J Bach

Abstract

1. Interactions between the fungicide prochloraz and the hepatic cytochrome P-450 of rainbow trout were studied by determination of enzymic activities in vitro using the microsomal fraction, and by kinetic studies. 2. Prochloraz inhibited 7-ethoxyresorufin-O-deethylase (EROD) in vitro. This inhibition was partially non-competitive: the enzyme-substrate-inhibitor (ESI) complex was catalytically active (68% of the activity of the enzyme-substrate complex). 3. Prochloraz in vitro inhibited aldrin epoxidase (AE) by a linear mixed-type mechanism. This inhibition might be high because of high affinity for prochloraz and lack of catalytic activity of the ESI complex. 4. Effects of prochloraz in vivo were studied as a function of time after intraperitoneal injection. Total cytochrome P-450 increased for more than 21 days. 5. EROD increased slightly at day 4 and then returned to control level. Kinetics showed an increase in apparent Km and Vmax. AE was strongly inhibited at day 4 (large increase in apparent Km) and then returned to control level in 21 days. 6. Spectral interactions with aniline showed strong inhibition and recovery to an activated level at day 21.

References

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Citations

Aug 26, 1998·Neuroscience Letters·R Vandame, L P Belzunces
Apr 6, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·S L Levine, J T Oris
Apr 24, 2007·Ecotoxicology and Environmental Safety·Daniel L VilleneuveMarkus Hecker
Jun 10, 2011·Environmental Toxicology·Inês DominguesSusana Loureiro
Jan 10, 2014·Toxicology Mechanisms and Methods·Buket AlpertungaGül Ozhan
Feb 2, 2017·Environmental Toxicology and Chemistry·Michele GottardiNina Cedergreen
Sep 19, 2017·Environmental Science & Technology·Andrea RöschJuliane Hollender
Nov 19, 2021·Chemical Research in Toxicology·Nadiia MosiichukVolodymyr Lushchak

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