Effects of the mannose, the major component of Lycium barbarum polysaccharides, and its potential target metabolite inositol on mouse β-TC6 cells
Wei sheng yan jiu = Journal of hygiene research
Xiangling ShiGuiju Sun
Abstract
To investigate the effects of mannose, the major component of Lycium barbarum polysaccharide, and its potential target metabolite, inositol, on mouse islet β-TC6 cells. Different concentrations(0, 4. 6875, 9. 375, 18. 75, 37. 5, 75 and 150 μg/mL) of mannose or inositol were used to intervene β-TC6 cells for 24 hours, and the proliferation activity of cells was determined by CCK-8 method. Enzyme-linked immunosorbent assay was used to detect insulin secretion after the intervention of the β-TC6 cells from different concentration of the mannose or inositol(0, 18. 75, 75 and 150 μg/mL) combining with glucose stimulation(20 mmol/L) for 60 minutes. Pioglitazone(3. 92 mg/L) was set up as positive group, and after intervention of the mannose or inositol(0, 9. 375, 18. 75, 75 and 150 μg/mL) for 24 h, the expression levels of insulin, glucose kinase(GK), glucose transporter 4(GLUT4) and glycogen synthase(GS) mRNA were detected by real-time quantitative PCR. Compared with the control group, mannose and inositol promoted the proliferation of β-TC6 cells in a concentration-dependent manner(18. 75-150 μg/mL)(P<0. 05). Although the inositol solution of 4. 6875 μg/mL and 9. 375 μg/mL had a tendency to promote cell proliferation, there was n...Continue Reading