PMID: 223674Jun 1, 1979

Effects of the medium on the rate of electron transfer in a reconstituted system of mitochondrial hydroxylation

A G ArmenianR M Nalbandian


Effects of ionic strength, pH, viscosity, concentrations of components and nature of acceptor on the rate of NADPH oxidation and acceptor reduction were studied in a hydroxylation system containing adrenodoxin reductase, adrenodoxin and cytochrome P450 or cytochrome c. The maximal rate was observed with 0.05--0.10 M phosphate buffer, pH 6.0--6.5 and at the adrenoxin/flavoprotein/cytochrome ratio of 1 : 1 : 1. The electron transfer rate was decreased with an increase in viscosity. Cytochrome P450 is more efficient as a terminal acceptor as compared to cytochrome c or indigodisulphonate.

Related Concepts

Cytochrome c Group
Cytochrome P-450 Oxygenase
Respiratory Chain
Ferredoxin-NADP Reductase
NADH, NADPH Oxidoreductases

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