Effects of the multilayer structures on Exenatide release and bioactivity in microsphere/thermosensitive hydrogel system

Colloids and Surfaces. B, Biointerfaces
Puxiu WangMingyan Jiang

Abstract

Traditional polypeptide-loaded PLGA microspheres (PM) using emulsion electrospray techniques often exhibit unsteady release and limited bioactivity. To solve these two problems, an Exenatide (EXT)-loaded multilayer system composed ofPM and thermosensitive hydrogel was prepared by the emulsion electrospray technique in this study. Hydrogel mixture were loaded in PLGA microspheres as Depot-hydrogel to prepare Gel/PM. The PM/Gel and Gel/PM/Gel systems were obtained by dispersion of PM and Gel/PM into hydrogel mixture, respectively. EXT in Gel/PM/Gel showed a constantly in vitro release for 30 days, which was significantly enhanced in comparison of those in the PM/Gel and the Gel/PM. PM/Gel and Gel/PM/Gel showed diminished burst release and no platform period compared with PM and Gel/PM. And these could be because the introduced Matrix-hydrogel outside, as a buffer layer, inhibited burst releases and exhibited a sustained manner. The inner Depot-hydrogelstructure slowed the PLGA degradation rate and drug release rate. As well, more than 15-day blood glucose levels in KKAy mice were greatly maintained at 7.50-9.50 mmol/L after a single subcutaneous injection of Gel/PM/Gel (4.95 μg/kg). Spatial stability and further bioactivity of re...Continue Reading

Citations

Oct 16, 2018·Frontiers in Chemistry·Guiting LiuZhipeng Gu
Mar 4, 2020·Environmental Science and Pollution Research International·Hongxue DuMingyue Piao
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Tingting YuBing Han
Mar 19, 2021·Biomacromolecules·Ana M Fuentes-CaparrósDave J Adams
Aug 22, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Xibang ZhaoJiali Wang

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