Effects of the mycotoxin deoxynivalenol on steroidogenesis and apoptosis in granulosa cells

Reproduction : the Official Journal of the Society for the Study of Fertility
Hilda M Guerrero-NetroChristopher A Price

Abstract

Mycotoxins can reduce fertility and development in livestock, notably in pigs and poultry, although the effect of most mycotoxins on reproductive function in cattle has not been established. One major mycotoxin, deoxynivalenol (DON), not only targets immune cells and activates the ribotoxic stress response (RSR) involving MAPK activation, but also inhibits oocyte maturation in pigs. In this study, we determined the effect of DON on bovine granulosa cell function using a serum-free culture system. Addition of DON inhibited estradiol and progesterone secretion, and reduced levels of mRNA encoding estrogenic (CYP19A1) but not progestogenic (CYP11A1 and STAR) proteins. Cell apoptosis was increased by DON, which also increased FASLG mRNA levels. The mechanism of action of DON was assessed by western blotting and PCR experiments. Addition of DON rapidly and transiently increased phosphorylation of MAPK3/1, and resulted in a more prolonged phosphorylation of MAPK14 (p38) and MAPK8 (JNK). Activation of these pathways by DON resulted in time- and dose-dependent increases in abundance of mRNA encoding the transcription factors FOS, FOSL1, EGR1, and EGR3. We conclude that DON is deleterious to granulosa cell function and acts through a RS...Continue Reading

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Citations

Dec 23, 2016·Asian-Australasian Journal of Animal Sciences·Hyejin ShinHyunjung Jade Lim
Feb 18, 2018·Journal of Pineal Research·Mei LanShao-Chen Sun
Apr 4, 2018·Journal of Applied Toxicology : JAT·Kinga Anna UrbanekAgnieszka Wanda Piastowska-Ciesielska
Jul 25, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Peng HanChristopher A Price
Nov 21, 2017·Archives of Toxicology·Qinghua WuKamil Kuca
Dec 24, 2019·Reproduction in Domestic Animals = Zuchthygiene·Min YangXiaodong Zhang
Nov 19, 2018·Reproduction, Fertility, and Development·David A LandryMarc-André Sirard
Nov 4, 2020·Journal of Applied Toxicology : JAT·Hilda M Guerrero-NetroYounes Chorfi

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